| Xanthophylls are members of carotenoids family, including lutein and zeaxanthin. As an excellent antioxidants, natural xanthophylls have some properties different from other carotenoids, such as protection of visual acuity, reductuction of incidence of cataract. Since the chemical synthesis process of xanthophylls is complicated and their corresponding biological activity is poor, almost all the commercial xanthophylls products are taken from natural compound. Corn Gluten Meal (CGM) is a by-product from corn starch manufacturing, in which the xanthophylls content is up to 145μg/g. Natural xanthophylls can be obtained from CGM, which has a great annual output.Xanthophylls were determined by HPLC using YMC-Carotenoid C30 column 4.6mm×150mm (3μm). Mixture of methanol, methyl tertiary butyl ether and water (95:2:3) was used as elution solvent with flow rate of 1.0mL/min. The wavelength of UV detector was 450nm/260nm and the column temperature was 30℃.The separation of xanthophylls from CGM mainly consists of three operation process including pretreatment, extraction and preparative separation. Comparing with the proteolytic hydrolysis pretreatment method, the surfactant pretreatment of CGM can enhance the extraction ability of xanthophylls by ethanol. Then in the pretreatment process the pigment was extracted by industrial alcohol for getting rid of the insoluble proteins and lipids. Before purification with column chromatography, the performance and separation characteristics relevant to xanthophylls were measured on HZ816,D630,SD300,S8,AB8 resins. It was found that SD300 macroporous adsorptive resins offer the largest adsorption abilities among the tested adsorbents and thus was selected as the stationary phase. Using Ethyl acetate as mobile phase, the content of xanthophylls in product was increased to 6.0%, with a recovery of 80%. |
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