| Folic acid(FA) is a water-soluble B vitamins, which is composed from Pteridine, p-aminobenzoic and acid glutamic acid, also known as vitamin BC or vitamin M. As a vitamin, folic acid is necessary organic compound for maintaining normal life processes. Folic acid as a coenzyme in life activity, which plays an important role in cell growth, reproduction and protein metabolism of the body. It is an indispensable material for red blood cells. Folic acid and vitamin B12 work together to promot red blood cells's formation and maturation. Pregnant women who lack of folate may lead to newborns's neural tube defects, and children may have no brains. Folic acid can not be synthesized in the body, mainly availabled from food. The body demand for a little folic acid, but indispensable.In current years, due to the importance for health, folic acid has become a focus in medicine, biology and chemistry's research. In natural samples, folic acid content is very low, composition is complex and stability is poor. These is a challenge for researchers to study. The number of researchers like Electrochemical method because it is be directly measured, not required separation and purification in the determination.In this article, Cyclic voltammetric behavior of folic acid on platinum nanoparticles/multi-wall carbon nanotube modified glassy carbon electrode (PtNPs/MWNT/GCE) has been studied with Cyclic voltammetrey. The modified electrode(i.e. PtNPs/MWNT/GCE) combines the features of platinum nanoparticles'excellent catalytic activity and multi-walled carbon nanotubes'large surface area, good electron transport properties. Multi-walled carbon nanotubes and platinum nanoparticles/multi-walled carbon nanotubes are characterized by transmission electron microscopy, found platinum nanoparticles attached to the multi-walled carbon nanotubes and disperse effect is great.The result indicates that folic acid can exhibit a very sensitive irreversible reduction peak at 0.95V (vs SCE) in pH=6.6 phosphate buffer solutions. The modified electrode exhibits a good promoting effect on the electrochemical reaction of folic acid. The electrode effective surface area is 0.136 cm2, the electron transfer coefficientαis 0.845. Experimental conditions include the electrolyte solution, the amount of multi-walled carbon nanotubes and platinum nanoparticles, scan rate, pH were optimized. Under the optimized conditions, the peak current is linear to FA concentration over the range of 2.0×10-71.0×10-4 mol/L by using differential pulse voltammetry, and the detection limit is 5.01×10-8 mol/L, with correlation coefficient of 0.997. The methods can be used for the determination of the drug in tablets, spinach, milk with good results. The samples of the correlation coefficient are 3.215.41%, the recovery rate was 95.7105.9%. PtNPs/MWNT/GCE is successfully applied to the determination of FA in real samples. |
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