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In-situ Bioremediation Of Complex Organic Polluted Groundwater By Bio-augmentation In Laboratory

Posted on:2012-01-21Degree:MasterType:Thesis
Country:ChinaCandidate:H J LiFull Text:PDF
GTID:2211330338958418Subject:Environmental Engineering
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With the rapid development of industry and agriculture, the phenomenon of groundwater by organic polluted has already common, especially toxic organic pollution more and more seriously. Organic pollutants which pollutes the groundwater mainly includes industrial raw materials, intermediate processed products, semi-finis hed products and finished products and so on. Because of the pollution, much groundwater resource was destructed and lose the exploitation value and drinking function. If people don't effectively repair and manage, it will produce a series of environmental problems and negative environmental impacts. Therefore, the topic based on the polluted groundwater of double benzene factory in Jilin City as the research object, simulated its groundwater environment indoor, and used the casting bacteria method to finish the situ bioremediation.The results showed that the polluted groundwater of double benzene factory in Jilin City was a comples pollution system. There were many kinds of pollutants which generated very serious pollution, for instant, the concentrations of nitrobenzene and aniline reached 194.40 mg/L and 92.72 mg/L respectively. The main goal pollutants which were confirmed finally were nitrobenzene, aniline, benzene, toluene and monochlorobenzene. On the condition of aniline and nitrobenzene worst polluted as the research objects, it found that natural sand didn't basically adsorb aniline and nitrobenzene.On the natural conditions of simulating polluted groundwater, nitrobenzene, aniline, toluene, and monochlorobenzene had certain self-purification phenomenon, but benzene almost had no attenuation trend. After dosing degradation bacterium and drumming wind aeration, nitrobenzene, aniline, toluene, and monochlorobenzene had sharp drop. The strainaway rates of nitrobenzene and aniline were more than 80.00%, and the effluent concentration was less than 2 mg/L. All substances were detected in effluent water, the result showed out that benzene was17.39μg/L, toluene was0.41μg/L, monochlorobenzene was0.73μg/L, and their removal rate respectively reached 88.62%,90.61%,90.24%. These datas indicated that dosing degradation bacterium and aeration could effectively perform complex remediation of ground -water polluted by organics.After stopping aeration, the concentration of nitrobenzene and concentration rose gtadually, their removal rate reduced from84.00% on the aeration to 74.00%. The effluent concentration of Benzene rose to 43.81μg/L, and its removal rate was reduced to 71.46%; The effluent concentration of toluene rose to 0.99μg/L, and its removal rate was reduced to 77.34%; The effluent concentration of monochlorobenzene rose to 1.21μg/L, and its removal rate was reduced to 86.46%.In the aeration stage, dissolved oxygen in the groundwater system was above 2 mg/L, it accounted for that the system was in good oxygen environment.In upstream areas, dissolved oxygen was on the average of 2 mg/L, and on the average of 6.5 mg/L in the downstream areas. The concentration of sulfate radical still has been steady status on the average of 230 mg/L; the concentration of nitrate radical in upstream areas was smooth about 15 mg/L equally, it increased dramatically and smooth in downstream areas on the average of 55 mg/L. After stopping aeration,the dissolved oxygen levels of the whole system dropped and evenly distributed, dissolved oxygen was within 2 mg/L in the mass. The concentration of sulfate radical occurred tiny changes as a whole between 10 mg/L and 30 mg/L; the concentration of nitrate radical reduced sharply to 1-4 mg/L.Activity of dehydrogenase in the whole system device existed certain differences along the direction of the underground flow. The activity of upstream areas was higher than that in middle and down stream areas,it stated that microbial biomass and biological activity in upstream areas was higher. In addition, it showed out that microorganisms has certain ability of transport and maintained a certain activity along the groundwater flow.
Keywords/Search Tags:adding microorganisms, in-situ, remediation, nitrobenzene, aniline
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