Study Of The Temperature Influence On Anammox

As a symbol of the New Nitrogen Removal Biotechnology,anaerobic ammonia oxidation process(ANAMMOX)has become one of the hottest research biological treatment problems both home and abroad. Some remarkable achievements had made in the mechanism,strain identification and the influencing factors of the ANAMMOX process. But, combination economic conditions in engineering application, the temperature factor are not researched deeply in such critical factors. In addition, how to preserve ANAMMOX sludge efficiently so that it remains a high activity has reported and studies similarly.Therefore, through controlling the temperature,no temperature control and recovering the temperature at last, I discusses the operational effect of several different temperatures on ANAMMOX reactor A in the laboratory, which the efficacious volume of 2.1L, basing on a continuous flow manner; In addition, by sequencing a batch experiment, I set different sludge conditions and the same preserved recovery conditions (influent NH₄⁺-N,NO₂^--N concentration were 100mg/L,130mg/L, pH was 7.3±0.2), explore the effect of different preservation temperature, concentration and time, and get a best preservation methods. The major contents were as follows:1) ANAMMOX reactor operating less than 26℃has a slightly lower effect than 37℃, and the effect will be restored after 15 days. If operating at 26℃can reduce the economic cost, it is recommended that constant temperature in the long run this ANAMMOX reactor instead of 37℃.2) The ANAMMOX reactor can operate between 22.7℃and 30.8℃for a long-term running suitably. When the ANAMMOX reactor operate between 24.2℃and 30.8℃, the effect of the reactor will be better as the operating temperature in a swell increase, but not proportional; but sudden drop in temperature of the reactor operation has a larger impact than ascending; small-scale temperature fluctuations or sudden changes in the operation of the reactor will not cause a greater impact. When the temperature rose to 30℃, the total nitrogen removal and nitrogen removal rate was higher than 37℃. When the ANAMMOX reactor runs stable, the capacity withstand temperature fluctuations more than 19℃will be greatly enhanced, the small size of the heating or cooling the reactor will not bring greater extent to running. Lower than 19℃is seriously affecting the operation of the reactor effect, and it is not suitable for a long-term operation. If the reactor runs at such a low temperature in a long-term, the result will be returned in a short time when it rises to 37℃.3) Different preserved temperature and time on the activity of ANAMMOX sludge has different influences. The most appropriate temperature of the ANAMMOX activated sludge is 15℃nearby under a short-term (less than 365 days) preservation, about 5℃takes the second place. Compared with other storage temperatures, the activated sludge preserved under the Room temperature, which the total nitrogen removal rate reaching 0.107 kg/(m³·d) has small attenuation index of 0.012, the half-life is 29 days. The total nitrogen removal rate will decrease with the time of its rate becomes slow. Preserved within 150 days after the recovery of the sludge after 15d culture, sludge nitrogen removal rate can reach more than 200%.4) Different concentrations have different suitable preserved time. The ANAMMOX sludge preserved under 2-10℃is suitable for preservation in the starvation of 30 days without any concentration, and preserved under of the total nitrogen sludge load of 531 Kg(SS)/Kg(N) for 45 days. The higher concentration of sludge and the more deposited sludge, the faster activity decreased, and a long time is needed returning to the previous activity preserved.5) Preserved under the same concentration, the color of ANAMMOX sludge will turn black with a higher temperature gradually; Preserved under different concentrations, the ANAMMOX sludge color changes thinly after short-term preservation (45d or less) in non-starved. The sludge color after preserved can not explain the level of activity, but the higher activity of ANAMMOX sludge after preserved, the shorter time the sludge required, returning to the color and activity before deposited. When the experiment ended, the sludge color after any state of preservation has significantly improved close to red brown.