The Research Of Embedding Pesticide Degrading Bacteria And The Performance Degradation

Carbendazim is a broad spectrum fungicide, it has been used in diseases prevention and treatment of various crops widely. Continuouly useing of carbendazim in the filed, so many plants were resistanted pathogens. Microbial remediation technology is a highly effective synthetic environmental technology. It transforms organic pollutants of macromelecule into inorganic mineral. It was not only completely degraded, but also easy to operate, with less remediation cost. In order to improve low microbial resistance to environment changes, shorten the processing time, embedding was required to improve the microbial degradation.Embedded conditions and degradation conditions of two carbendazim degrading bacteria were discussed in this paper. The following were obtained:For Alcaligenes sp. bacteria, the optimal SA embedding condition is that the bacteria were mixed with 2% SA and cross-link in 4% CaCl₂ for 24h at room temperature. The optimal PVA embedding condition is that the bacteria were mixed with 10% PVA and cross-link in 3% CaCl₂ under the same condition. The optimum temperature is 30℃, the degradation rate of free bacteria is 63.6%, SA embedding degradation rate is 81.5%, PVA embedding degradation rate is 73.3%. The best reaction pH is 6, the removal rate of free bacteria is 52.5%, SA embedding is 76.8%, PVA embedding is 78.9%. 2:10 to determine the highest removal rate of carbendazim as the best inoculation. Under the best inoculation, SA embedding carbendazim removal rate is 75.8%; PVA embedding is 78.1%. When carbendazim concentration is 100mg / L, the free bacteria degradation rate of carbendazim 91%, SA and PVA embedding can remove completly. the best activate time is 48h. Under these conditions, SA embedding removal rate is 78.2%, PVA embedding removal rate is 80.3% .For Rhodococcusery thropolis bacteria, the optimal SA embedding condition is that the bacteria were mixed with 3% SA and cross-link in 3% CaCl₂ for 24h at room temperature. The optimal PVA embedding condition is that the bacteria were mixed with 10% PVA and cross-link in 3% CaCl₂ under the same condition. The optimum temperature for bacteria degrading carbendazim is 30℃, the degradation rate of free bacteria is 62.7%, SA embedding degradation rate is 82.8%, PVA embedding degradation rateis 87.1%.The best reaction pH is 6.5, the removal rate of free bacteria Carbendazim is 56.1%, SA embedding degradation rate is 76.7%, PVA embedding degradation rate is 78.2%.2:10 is the highest removal rate of carbendazim as the best inoculation. Under the best inoculation, SA embedding carbendazim removal rate is 83.4%; PVA embedding carbendazim removal rate is 78%.When carbendazim concentration is150mg / L, the free bacteria is in the degradation rate of 93.4%, SA embedding degradation rate is 97.5%, PVA embedding degradation rate is 98.2%. The best activating time is 48h. Under these conditions, SA embedding removal rate is 80.2%, PVA embedding removal rate is 83.1%.The Alcaligenes sp. Bacteria and Rhodococcusery thropolis bacteria are bacterias, condition impact have the same law, two embedding methods also have shown the similar impact changes.