Methods For Lead Analysis In Blood, Urine And Soil Samples

Our entire life support system is dependent on the well-being of the environment. Pb as a consequence of industrial activities is well kown a very hazardous contaminant. The Pb pollution control and investigation has already become one of the key issues in environmental science and technology. Toxicological properties of Pb depend on various species present in environment. Speciation analysis has been paid attention to, and developed rapidly decades. The distribution, migration, transformation, bioavailability and toxicity of elements are species. Thus, the total analysis and characterization of lead species in samples is an important pursuit. In this dissertation, the trends of amount of Pb element in blood and urine of lead-exposed pigs was determined by inductively coupled plasma mass spectrometry. A method based on high performance liquid chromatography coupled with inductively coupled plasma mass spectrometry for simultaneous determination of organolead complexes was established.(1) The trends of amount of Pb element in blood and urine of lead-exposed pigs was determined by ICP-MS. The detection limits for Pb was 0.08μg/L and its regression coefficient was 0.9999. The method was verifyed by analyzing the Certified Reference Materials(CRMs), such as GBE09140 and GBW09105a. The Pb concentration values obtained for CRMs closely matched the certified values and their RSD were below 3.39% . The recoveries for blood lead and urine lead were 86.0%-94.5% and 87.0-98.0% respectively. Pb elements in blood and urine of lead-exposed pigs in different period of time were analyzed with the new established method. The results of the analysis indicate that the blood lead of two kinds of lead-exposed pigs increased to 300μg/L and 230μg/L, at 5h respectively, and decreased to 300μg/L and 230μg/L at 72h. While urine lead of two kinds of lead-exposed pigs reach to 180μg/Lå'Œ130μg/L at 24h respectively, and decreased to 110μg/Lå'Œ50μg/L at 72h..(2) A method based on HPLC-ICP-MS for simultaneous determination of organolead complexes (Trimethyllead (TML), Triethyllead (TEL) and TriphenylLead (TPhL)) was established. After evaluation of different additives of separation condition, including Mobile phase composition, chromatogram column, elution mode, flow speed from 0.15mL/min to 0.4mL/min and pH from 4.8 to 4.3, the method allowed the best chromatographic separation of the three compounds. The method detection limit for TML, TEL and TPhL is 0.14μg/L,0.33μg/L and 1.45μg/L respectively, and their RSD were 4.6%, 5.5% and 2.4%. The recoveries for TML and TEL were 101.9% and 98.8%, but the method had established can not extract TPhL.