Derivatization Of Animo Acid In Beer Yeast Zymolysing And Preparation Of Meat Flavour

With beer yeast as raw material, zymolysing fluid including amino acids can be prepared by enzymolysis. The amino nitrogen actual recovery was 4.04%, soluble content was 9.6%,product yield was 63.9%.By phthaldialdehyde column derivatization we got the main amino acid contents:glutamate 1.85 mg/mL; serine 1.32 mg/mL; threonine 1.81 mg/mL; arginine 1.32 mg/mL; alanine 1.70 mg/mL; valine 1.93 mg/mL; phenylalanine 1.49 mg/mL; leucine 2.29 mg/mL; lysine 7.11 mg/mL; isoleucine 1.86 mg/mL. Compared of amino acid analyzer, HPLC-OPA precolumn derivation method has lower requirement of instrument, better separation effect and broader application prospects.Taking adsorption rate and exchange rate into account, compared 732,WA-2,330 resins and so on, by ion exchange method to extract amino acids contained in zymolysing fluid, considering adsorbing rate and exchanging rate, chose WA-2 ion exchange resin to separate the amino acids in zymolysing fluid, confirmed the best condition:pH= 6-8, used 0.04mol/L ammonia as elution agents, through the thin layer chromatography qualitatively and HPLC it is obtained that the purity was 90.2%.Through silica gel column chromatography to separate the zymolysing fluid and used the elution agents like this:n-butyl alcohol:glacial acetic acid:H2O=4:1:1(V:V:V). Amalgamated the same Rf value components, then removed the solvent, by high-performance liquid detection can get a conclusion, the component which Rf value was 0.57 had the higher purity. By using the retention time and Rf value can infer the component was valine. The purity of valine was 93.56%.Used beer yeast and reducing sugar as raw material to produce Maillard Reaction, and then took an organoleptic evaluation for the reaction products. By single factor experiment the reaction main additives amount was confirmed:glucose 3g, xylose 2g, taurine 2g, cysteine 3g, zymolysing fluid 50g,H2O 50g, lard oil 5g. Through orthogonal experimental method to optimize the reaction time, reaction temperature, pH value, the optimum condition was got like this:reaction temperature 100℃, reaction time 60min, pH=5.