The Toxicity Of Phenanthrene And Sodium Percolorate To Zebrafish

Due to its toxicity, durability and severe harmfulness to human health and entironment, persistent pollutants have got attentions since the recent dacades. Using adult zebrafishs and its embryos as test organism and considering the current state of environmental pollution domistic and overseas, Phenanthrene existed in persistent organic contaminants and sodium perchlorate in persistent inorganic contaminants are selected as objects of study. This paper mainly studied on the single acute toxicity effects of the two objects to the adult fish as well as the embryos, and the first time study the joint toxicity effects of the two objects to the adult fish, thus provides scientific evidence for accurately evaluating the ecological security of the two compounds.This paper according to Water quality-Determination of the acute toxicity of substance to a freshwater fish do the acute toxicity experiment, the result indicates that, the 96hLC₅₀ of the phenanthrene and the sodium perchlorate to the adult zebrafish are 0.74mg/L and 3352.8mg/L. Phenanthrene belongs to medium cumulative toxicity. The joint toxicities of the phenanthrene and the sodium perchlorate to the adult fishs indicates that, when toxic unit ratios is 1:1, the 96h-LC₅₀ of phenanthrene is 0.15mg/L, sodium perchlorate is 1562.8mg/L; when toxic unit ratios is 1:1, the 96hLC₅₀ of phenanthrene is 0.35mg/L, sodium perchlorate is 2398.8mg/L; when concentrations ratios is 1:1, the 96hLC₅₀ of phenanthrene is 0.35mg/L, sodium perchlorate is 0.79mg/L。Using the Brachydanio rerio embryo development technique, the single acute toxicity of the two compounds to the embryos results in that, with the rise of exposure concentration, the malformation rate and mortality of the embryos caused by the phenanthrene and the sodium percolorate increase, while the heart rate and hatching rate decrease. The 96h EC₅₀ of phenanthrene is 0.33mg/L, which is 3972mg/L for the sodium percolorate. The activity of SOD was being restrained at the beginning but induced in the later stage.The research shows that, phenanthrene is a compound of a high toxicity and has a 96hLC₅₀ of 0.74mg/L to the adult fish. Sodium percolorate is a compound of a low toxicity and has a 96hLC₅₀ of 3352.8mg/L. The two compounds can cause the rise of the death and deformity rates, as well as the decrease of the heart and hatching rates to the embryos. The phenanthrene and the sodium percolorate have a joint toxicity of antagonism.