Preparation And Antioxidative Activities Of Wheat Germ Peptides Of Helongjiang

In this study, small molecules of wheat germ peptide was extract by extracting protein of wheat germ, protease, ultrafiltration purification.The refined wheat germ peptide was analysis by functionality.molecular weight distribution, anti-oxidant activity,and acid composition. The nutrient beverage recipe of wheat germ peptide was also studiedUsing method by alkaline extraction and acid precipitation of wheat germ protein extraction, through the analysis of single factor and orthogonal to determine the optimum extraction of wheat germ protein was:pH was 10.00, extraction temperature was 50℃, extraction time was 90mins, solid to liquid ratio was 1:25, the best extraction rate of wheat germ protein was 78.70%.According to data defining the DH and the antioxidative activities,wheat germ peptides were prepared by singal- proteases hydrolyzed, mixed double- proteases hydrolyzed simultaneously and double- proteases hydrolyzed separately in steps. The results showed that the singal- proteases optimum hydrolytic condition for antioxidative activities was alcalase;double- proteases hydrolyzed separately in steps was better than mixed double-proteases hydrolyzed simultaneously, the optimum hydrolytic was added alcalase ealier than papain.The scavenging rate of Dpph of wheat germ peptide by ultra-filtration was separated by UF of 3kDa and 6kDa, the molecular weight was less than 3kDa had the best scavenging rate of Dpph,which was 75.32%.The optimal ultra-filtration parameters were determined by response surface analysis,the results show ultra-filtration pressure was 0.08Mpa, ultra-filtration time 41.37min, ultra-filtration pH was 6.92, ultra-filtration concentration was 2%,the flux was 4.85 L/(m2·h)The wheat germ peptide after refined was studied in vivo and in vitro antioxidant function,.The results show that wheat germ peptides have a very good free radical scavenging capacity, inhibition of lipid oxidation, and could improve the aging mice antioxidant capacity.By determined amino acids of wheat germ Peptide,measured the peptides of wheat germ contained the amino acid was suitable for the body to absorb.The optimmrecipe of wheat germ peptide nutrient beverage was developed from orthogonal experiment and uniform design experiment.The best recipe was peptide of wheat germ 5.00%, honeyl.75%, sucrose7.50, Citric acid0.2, Ethyl maltol 0.003%, CMC0.01%, xanthan gum0.06%, pectin0.01%.