Oxidation Damage Induced By DEHP On The Cells Of Esisenia Foelide And Distributions Of DBP In The Rat Organs

This study was divided into two parts. The first part was to study the oxidative damage induced by di-(2-ethylhexyl) phthalate (DEHP) and find the possible effects of it. We use Esisenia foelide for the experimental material to study the biomarker DNA-protein crosslinks (DPC), the contents of malondialdehyde (MDA), and the activity of surperoxide dismutase (SOD). We made the Esisenia foelide as experimental material exposed to DEHP for 2 hours. The results showed that, the DPC coefficients were increased along with the concentration of DEHP (0.2 mmol·L-1,0.4 mmol·L-1,0.8 mmol·L-1,1.6 mmol·L-1) rising, compared with the control group (p<0.01), the contents of MDA did not change significantly with the concentrations of DEHP compared with the control group (p>0.05). The activities of SOD decreased with concentrations of DEHP rising. There are significantly changes exit in 0.4,0.8 and 1.6 mmol-L-1 (p＜0.01) groups compared with control group. The second part was to check out whether the DBP can stay in body for over 24 hours, we used the Wistar rats as experimental material for this study, exposed to DBP through mouth for one week, then check the contents of DBP in the kidney, liver, stomach, testis after 24hours. The results shows, there are many DBP in the kidney and liver and little in the stomach and the testis.