Research About Photopolymerizationable Bionic Bioadhesive

Bioadhesive is well known as medical material with a certain biocompatibility and adhesion applied to biological tissue.Photopolymerization technology applied to the bioadhesive as a new research direction, few reports related to this research. When compared with other method, photopolymerization technology has a many advantages such as faster curing, less damage to organism, more widely range of sources of the monomers and resin than a-cyanoacrylate adhesives and the fibrin sealant. Especially for the irregular site of injury, this method is easier to operate.This research inspired by the mussel adhesion protein having the superior water-resistance bonding capacity. Choosing the dopamine (containing catechol groups) as the adhesive functional group, the monomer DMA with single light-sensitive functional group and EGAMA-DOPA with double light-sensitive functional groups was prepared. The structure of products was analyzed by using the FTIR and NMR; and the photopolymerization conditions of above monomer were researched, such as initiator and monomer concentration, light intensity, and solution composition etc. The gel time was characterized by series real time near infrared spectroscopy (SRTIR). And the swelling behavior of gel, bond strength, bursting pressure and in vitro biocompatibility were also studied. In addition, the modified light-sensitive chitosan was prepared and used for cross-linking DMA gels. At the same time, in order to improve the adhesion of the elecrospun membrane, we introduced the DMA into the nanofibrous membrane. The nanofibrous membranes would be further applicable for skin regeneration.The Details and conclusions are described as follows:1, The samples with different composition, each of them could complete gelation within 3-15min. The adhesive strength of the samples adhered to gelation sheets to simulate the living tissues could reached 3.5MPa. The highest burst pressure reached 178mmHg, when the samples adhered to fresh mouse skin. And good capability of the DMA gels supporting the mouse fibroblast (L929) attachment and proliferation are observed.2, Compared to the DMA, EGAMA-DOPA is a liquid. So it could be directly photocured by the visible light. In order to improve the performance of the gels, PEGDMA was introduced into the visible light curing system. As the results showed, most of the samples can be completed curing within 15min. In addition, we found the introduction of PEGDMA could not only accelerate the photopolymerzation and make the photopolymerization more completely, but also prevent the monomers dissociate, improve the biocompatibility and make the material toughness better.3, Dopamine methacrylamide/poly(ethylene oxide)(DMA/PEO) nanofibers were successfully prepared by electrospinning of aqueous DMA/PEO solution. Biocompatible nanofibrous membrane with good adhesion was produced by photocuring from the DMA/PEO nanofibers. This method provides a new idea for the preparation of the compound of multo-layer elecrospun membranes.