Determination Of Small Biomolecules And Drug Molecule By Acylpyrazolone Composite Film Modified Glassy Carbon Electrode

Determination of small biomolecules and drug molecule by single acylpyrazolone film or composite film modified glassy carbon electrode were investigated in this paper.The electrochemistry properties of A was tested on HPMαFP GCE. Compared to the bare electrode, the modified electrode showed an excellent electrocatalytical effect on the oxidation of A and displayed a shift of the oxidation potential in the negative direction with significant increase in the peak. Under the optimum conditions, linear calibration curve was obtained within the concentration range 5x10-7 to 1×10-4 mol/L. The detection limit was 1.25×10-7 mol/L. This developed method had been applied to the direct determination of A in tablet samples with satisfactory results.The novel modified electrode HPMαFP/MWNTs/GCE was used to determine the electrochemistry properties of isoniazid. The results showed that the composite film modified electrode showed an excellent electrocatalytical effect on the oxidation of isoniazid with significant increase in the peak current. The above results indicated that a synergistic effect was existed between MWNTs and HPMaFP. Under the optimum conditions, linear calibration curve was obtained within the concentration range 5×10-6 to 8×10-4 mol/L. The.detection limit was 1×10-6 mol/L. This method was applied to the direct detection of isoniazid in tablet samples with satisfactory results.The novel modified electrode HPMaFP/MWNTs/GCE was also used to determine the electrochemistry properties of XN in the presence of an excess of uric acid (UA). Compared with the bare GCE, the electrochemical sensor greatly enhanced the oxidation signal of XN with negative shift in peak potential about 110 mV. Based on this, a sensitive, rapid and convenient electrochemical method for the determination of XN has been proposed. Under the optimized conditions, the oxidation peak current of XN was found to be proportional to its concentration in the range of 0.3μM～50μM with a detection limit of 0.08μM. The analytical utility of the proposed method was demonstrated by the direct assay of XN in urine samples and was found to be promising at our preliminary experiments.