Investigation Of Community Structure Of Ectomycorrhizal Fungi In Abandoned Copper Mine And Effects Of Colonization Of Ectomycorrhizal Fungi On Growth Of Tree Seedlings In Copper Tailings

Ectomycorrhizal fungi (EMF), a large class of mycorrhizal fungi, are ubiquitous in forest ecosystem. These fungi can form mutually beneficial symbiotic relationship with host plants. The symbiosis improves host performance by enhancing nutrient and water uPsake from the soil, playing an essential role in host's colonization, growing and tolerance. This thesis investigated the EMF communities structure in a copper mine and examined the growth of pine and oak seedlings inoculated with different EMF in a copper mine tailing.To investigate the EMF communities structure in a copper mine, the roots from pine trees and oak trees were sampled in an abandoned mine (Mt. Jiuhua copper mine area), and the roots from random trees were also sampled in control area. ECM root tips were indentified for species-level identification. Results indicated that infection rates of EMF in mine were lower than those in control area. There were 11,8,8 of EMF species indentified from pine trees, oak trees and control area, respectively. Shannon's index and Simpson's index of those above EMF community indicated that the difference between each EMF community was not significant. Only a few of shared EMF were found among different invesitiged area, i.e.,2 between pine trees and oak trees,3 between pine trees and control area, and 3 between oak trees and control area. Moremove, the S(?)rensen similarity index between each community was also low. These results indicate that the EMF structure between each community was remarkablely different.To examine the growth of pine and oak seedlings inoculated with different EMF in a copper mine tailing, seedlings of pine or oak were inoculated with one of the three EMF species(Pisolithus sp.; C. geophinum; L. laccata), and then were transplanted into copper tailing (Mt. Donggua, Anhui province). The survival rates of pine seedlings infected by different EMF were significantly higher than those of control seedlings, but this trend could not be found in oak seedlings. The increment of fresh weight and shoot height for ECM pine seedlings were significantly higher than those of control seedlings. The difference in biomass increment of oak seedlings between ECM and control treatments was not statistically significant. EMF inoculation treatments can effectively increasing the Cu concentration in pine seedling, however, there were no significant effect of EMF inoculation on Cu absorption. Nutrients determination showed that EMF inoculation could enhance K and Ca uptake on pine seedlings, but this trend were not apparent on oak seedlings. Effects of all EMF inoculation of pine and oak seedlings on Mg uptake were not statistically significant. All of ECM roots in seedling root systems were senescent and dead after 7 month transplanting into the copper mine tailing.This study provides the theoretical basis for selecting heavy-metal stress EMF species and using EMF in ecological restoration on heavy-metal contaminated area.