Effect Of Different Culture Condiction On Toxic And None-toxic Microcystis Aeruginosa

Microcystis aeruginosa is the major of the algae species, and Microcystis aeruginosa can be divided into toxic and non-toxic Microcystis aeruginosa. Different environmental conditions can affect the growth rate of the toxin and non-toxic Microcystis aeruginosa. Several DNA extraction method were compared to establish an appropriate standard curve for the detection of toxic and none-toxic Microcystis aeraginosa. Real-time PCR was used to detected the concentration of the toxic and none-toxic Microcystis aeruginosa respectively. Plackett-Burman design was used to select out three significant growth factors,and then a central-composite design (CCD) for three independent variables, each at five levels, was used to design the experiment to investigate the individual and interactive effects on the toxin Microcystis aeruginosa accounted for a proportion of the total Microcystis aeruginosa.The experimental results were as follows:(1) Compared the DNA extraction effect between SDS lysis method,DNeasy Blood & Tissue Kit method and CTAB lysis method, the results showed that the DNA extracted by DNeasy Blood & Tissue Kit had better reproducibility than SDS lysis method and CTAB lysis method.(2) The standard DNA were extracted by SDS lysis method and DNeasy Blood & Tissue Kit respectively and amplified by real-time quantitative PCR.The results showed that the standard DNA extracted by DNeasy Blood & Tissue Kit were reproducible and could apply to detect the concentration of the toxic and none-toxic Microcystis aeruginosa respectively.(3) The individual and interactive effect of growth factors such as light intensity, temperature, PH, nitrate concentration, phosphate concentration were investigated by PB design and CCD design.Real-time PCR was used to detected the concentration of the toxic and none-toxic Microcystis aeruginosa respectively. Plackett-Burman design was used to select out three significant growth factors,and then a central-composite design (CCD) for three independent variables, each at five levels, was used to design the experiment to investigate the individual and interactive effects on the toxin Microcystis aeruginosa accounted for a proportion of the total Microcystis aeruginosa.The results showed that the effect of the light intensity and the nitrate concentration on the competition of the toxin and none-toxic Microcystis aeruginosa was significant, moreover, the effect of the light intensity was more significant than the nitrate concentration. The effect of temperature on the competition of the toxin and none-toxic Microcystis aeruginosa was not significant.