Application Of Cyclodextrin Functionalized Graphene Modified Electrode In Determination Of Anti-cancer Drugs And Nitrophenol

Chapter1:This chapter briefly introduced the properties, structure and the research progress of graphene. The research on pharmacology, toxicology and analytical detection and the electrochemical determination progress of doxorubicin, methotrexate and o-nitrophenol were summarized in this chapter.Chapter2:This chapter mainly introduced the synthesis of β-CD modified graphene (CD-GNs) from graphite and β-CD by wet chemical method. UV, IR, TGA, XPS, Raman, AFM and EIS were used to charaterize the prepared CD-GNs. The results showed that P-CD was connected to graphene surface, which made the CD-GNs had good dispersion in aqueous solution and good electrochemical behavior. CD-GNs can be applied to the electrochemical sensor.Chapter3:This study showed that the response current of DOX obtained on the CD-GNs modified glassy carbon electrode (CD-GNs/GCE) increased by26.5times compared to the result obtained on bare GCE. Under the optimized conditions, the new determination method of doxorubicin was established. There were a pair of redox peaks at Ep=-0.682V(vs.SCE) in0.1mol·L^-1 PBS (pH=8). The linear range was10nM～0.2μM,the linear equation was ip (μA)=18.0+236C (μM)(r=0.9981) and the detection limit of DOX was0.1nM (S/N=3), RSD=3.9%(n=6). Recovery experiment was carried out in urine, the recovery was97%～102%,which indicated that this method could be applied in detection of DOX in urine.Chapter4:This chapter studied the electrochemical behavior of methotrexate (MTX) on CD-GNs modified glassy carbon electrode. Under optimized conditions, the response current of doxorubicin obtained on the CD-GNs/GCE increased by23.7times compared to the result on bare GCE, the new determination method of MXT was established. There were a pair of redox peaks at Ep=-0.596V(vs.SCE) in0.1mol·L-1PBS (pH=6). The linear range was0.1～1.0μM, the linear equation was ip (μA)=1.29+67.8C (μM)(r=0.9994), the detection limit of doxorubicin was20nM (S/N=3), RSD=4.7%(n=6). Recovery experiment was carried out in urine, the recovery was100%～105%, which indicated that this method could be applied in detection of MTX in urine.Chapter5:This chapter discussed the electrochemical behavior of o-nitrophenol (O-NP) on CD-GNs/GCE by cyclic voltammetry (CV). Under the scan rate of0.05V·s-1, The results showed that after accumulation for10min, there were a pair of obvious redox peaks in0.1mol·L-1PBS (pH=7). The linear range was5.0～400μM, the linear equation was ip (μA)=0.1670+0.4316C(μM)(r=0.9997), the detection limit of O-NP was0.5nM(S/N=3), RSD=4.1%(n=6). This method showed good repeatability and could be used in the detection of O-NP in the sample of waste water.