GC-MS Based Metabolomics Approaches To The Mechanism Research Of Saccharomyces Cerevisiae Irritability To Ethanol

After industrial revolution, mankind is still of types of rapid-rich,and the demand for energy growth rapidly. Seeking for renewable cleanenergy becomes more and more important. Technics of fermentationethanol by yeast is an economical and environmentally friendly way toproduce renewable energy. While there are some limitations, during thefermentation process, Saccharomyces cerevisiae would be poisonedthrough this way. Gradually, the cells themselves, the shape and functionof cellular organ would also change. When ethanol concentrationaccumulated to a certain extent, which is more than the tolerance limit ofmore than yeast, the yeast will die. Therefore, understanding the impactof understanding of ethanol stress on the metabolism of Saccharomycescerevisiae stress mechanisms are particularly important.The research starts from the physiological condition of theSaccharomyces cerevisiae, four stages of its growth were measured, and achemical method was employed to determine the accumulated ethanol content. At ethanol stress point time, soak the stress of yeast cells withdifferent concentrations of ethanol (0%,3%,6%,9%,12%,15%), andmesure cell death rate and size. It was found that, the experimental strainsfor stability of long, accompanied by a slow secondary growth, couldbarely see the death of. Determine the stress time for eight hours in thelogarithmic phase. It was also found that, mortality don't increase as theethanol concentration linear growth, but a sudden increase after a certainconcentration is reached. As the ethanol concentration increased, the cellsize gradually changes, getting smaller and smaller.Secondly, by gas chromatography-mass spectrometry method, thestress of different concentrations of intracellular metabolites wasanalysised. Using principal component analysis (PCA, principalcomponent analysis) and partial least squares method (PLS, the partialleast squares), the composition of the six metabolites was get, andidentified22kinds of different metabolites.Finally, by using cluster analysis and metabolic differences in theexpression analysis of these differences are material integrated into themetabolic pathway of Saccharomyces cerevisiae in the analysis show thatthe proline in Saccharomyces cerevisiae respond to ethanol stress, stressreactions play an active role in the serine threonine, glycine, tyrosine, andamino acid metabolism plays an important role in stress mechanisms of S.cerevisiae on ethanol. In this study, metabolic pathway was analyzed by metabolomicsapproach, analysis of the stress mechanism of yeast in ethanol stress wasset from different angles. This study provides a theoretical foundation forfuture research.