Effects Of Four Kinds Of Chinese Herbal Polysaccharides On The Growth Performance, Body Composition, And Part Of Physiological And Biochemical Indicator In Yellow Catfish(Pelteobagrus Fulvidraco)

In this article, the yellow catfish was selected, and the effects of four kinds of Chinese herbal polysaccharides on growth performance, body composition and part of physiological and biochemical indicators of fish were studied. In addition, we discussed the effects of four kinds of Chinese herbal polysaccharides on the activity of immunologic cellular of yellow catfish.1. Fed diets with the different levels(300 mg/kg, 600 mg/kg, 900 mg/kg, 1 200 mg/kg, 1 500 mg/kg) of Astragalus polysaccharides(APS), Lycium barbarum polysaccharide(LBP), Lentinan(LNT) and Ganoderma lucidum polysaccharides(GLP) for 8 weeks, the growth performance and body composition of yellow catfish were analysed. The results showed that fed with 1 200 mg/kg LBP was the best effects on increasing the weight gain rate(P<0.01), length growth rate(P<0.05), the contents of protein in the muscle(P<0.01), and declining feed conversion ratio. The other three polysaccharides did not affected significantly on the growth performance, declining feed conversion ratio, and the contents of water, crude fat, crude ash and nitrogen-free extract in the muscle(P>0.05). GLP in the four polysaccharides, which had the best promotional effects on the contents of amino acids and essential amino acids, however there was not significant effects (P>0.05).2. In this study, GLP significantly enhanced the activities of protease, amylase, lipase in digestive tissues of yellow catfish, further more, the best adding level was 1 500 mg/kg. The diet fed with 1 200 mg/kg APS significantly enhanced the contents of total protein and albumin in the serum. Kinds and their optimum levels of polysaccharides in enhancing the contents of immunoglobulin, decreasing the contents of triglyceride and cholesterol in order were respectively 1 200 mg/kg of GLP, 1 500 mg/kg of LNT, and 1 500 mg/kg of GLP.3. After determining the effects of the four kinds of Chinese herbal polysaccharides on the SOD activities of yellow catfish, we found the order of each polysaccharide on enhancing the activities of SOD was LBP>GLP>LNT>APS, and 900 mg/kg of LPB had the best effect. The order of the four kinds of polysaccharides on CAT activities respectively was APS>GLP>LBP>LNT, and the effect of 1 200 mg/kg of APS was the best. The effects of decreasing the contents of MDA was APS >LBP>GLP>LNT, and the best one was 1 500 mg/kg of APS. The antioxidant effect of the four kinds of herbal polysaccharide had focus on different indicator, and 900 mg/kg of LBP, 1 500 mg/kg of GLP, 1 200 mg/kg of LNT, 1 200 mg/kg of APS had better antioxidant effect.The effects of four polysaccharides on the contents of NO had big differences, the order was GLP>LBP>LNT>APS, and we confirmed 1 500 mg/kg of GLP was the optimum level in promoting the contents of NO of yellow catfish by comparison.4. The four kinds of Chinese herbal polysaccharides could significantly improve the non-specific immune response of yellow catfish, but the emphasis was different. The optimum level of polysaccharides in improving the activity of phagocytic, the activity of lysozyme and pathogen resistance in order was 900 mg/kg of GLP, 1 200 mg/kg of APS and 1 500 mg/kg of GLP, respectively.5. The four kinds of Chinese herbal polysaccharides could improve the oxygen and nitrogen respiratory burst activity of kidney macrophage and the multiplication capacity of leukocytes in peripheral blood, and the optimum levels and kind of polysaccharides in order were 1 200 mg/kg of GLP, 1 500 mg/kg of LNT and 1 200 mg/kg of GLP; In conclusion, we confirmed fed diet with 1 200 mg/kg of GLP was the optimum in improving the immunologic cellular activity of yellow catfish.