| In recent years, due to the rapid development of transgenic technology , transgenic plants have been planted all over the world. It is refer to many characters,including insect resistance, anti-virus, anti-fungal, anti-herbicide, resistance adversity, quality improvement, and the regulation of growth and development to improve crop yields and so on. However, the safety of transgenic plants is focused on more and more by the society. It mainly consists of competitive of transgenic crops,transgenic on biodiversity, soil environment and nutrient recycling and so on.As the changing situation of the global climate environment, drought has already become the influential problem to plants'normal growth and development. In order to solve the major problems, transgenic DREB3 drought resistant soybean was bred successfully.Before commercialization and planting of transgenic drought resistant soybean ,environmental safety evaluation plays an important role to ensure the safety of the species in production. The thesis simulated the soil environmental aspects for transgenic DREB drought resistant soybean, and studied the influence of t he activity of soil enzymes and the soil functional microbial, to provides the referencefor establishment safety evaluation Method drought of root exudates of transgenic DREB3 drought resistant soybean on soil microbial activity and function microbial.The results were as follows:1. Under the normal soil water management, transgenic DREB drought resistant soybean has no notable effect on the rhizosphere soil urease, cellulose enzyme, phosphatase and hydrogen enzyme activity. Under the drought, urease activity of transgenic soybean was significantly lower than the receptor soybean on VE and R1,it renewed nomal at R8. Cellulase activity of transgenic soybean was significantly lower than the receptor soybean on VE ,R1and R4,it renewed nomal at R8. Phosphatase activity of transgenic soybean was significantly higher than the receptor soybean on R1,but was significantly lower than the receptor soybean on VE and R4.it renewed nomal at R8. Transgenic drought resistant soybean had no effect on soil hydrogen peroxidase activity. Shows that changes of soil urease, cellulase and phosphatase activity is directly related to drought stress.2. Separation a strain of ammonifying bacteria from rhizosphere soil of transgenic drought resistant soybean , was identified as Bacillus subtilis [Bacillus subtilis (Ehrenberg) Cohn], and named N. Separation a strain of cellulolytic microbes from rhizosphere soil of transgenic drought resistant soybean , was identified as Bacillus pumilus(Bacillus pumilus Meyer and Gottheil), and named C .Separation a strain of phosphobacteri from rhizosphere soil of transgenic drought resistant soybean , was identified as Bacillus megaterium (Bacillus megaterium), and named P.In normal soil water management, transgenic DREB drought resistant soybean has no significant influences on the number of rhizosphere ammonifying bacteria, cellulolytic and phosphobacteri. Under the drought stress, soil Bacillus subtilis population of transgenic soybean was significantly lower than the population of receptor soybean on VE.It was no different rest period. The soil Bacillus pumilus population of transgenic soybean was significantly lower than o receptor soybean on the R4, It was no different rest period. The number of phosphobacteri of transgenic drought resistant soybean was significantly lower than normal water management in VE. The number of Bacillus megaterium was no different of receptor soybean. Effert of transgenic drought resistant soybean on the number of the ammonifying bacteria, cellulolytic microbes and phosphobacteri is directly related to drought stress.3. Under the situations of the same root exudates of receptor soybean and drought resistant soybean, there were no significant difference in diameter functions of three kinds of soil microbial colonies.4. The same root exudates of transgenic and receptor soybean have no effect on the activity of soil enzymes of soil functional microbial. But three different root exudates of soil enzymes activity of soil functional microbial are different due to the different root exudates. Root exudates significantly activate the Urease activity of Bacillus subtilis and the phosphatase activity of Bacillus megaterium. When the concentration of root exudates of two kind of soybeans at 5‰, and concentration of root exudates of transgenic soybean at 0.05‰, cellulase activity of Bacillus pumilus was not difference, when the concentration of root exudates of two kind of soybeans at 50‰, and the concentration of he root exudates of receptor soybean at 0.05‰, the cellulose activity of Bacillus pumilus was inhibited significantly. |
PDF Full Text Request