Studying On Cloning And Silencing Of BraSDG8 Gene In Brassica Rapa L.

Breeding premature rapeseed is a new tough task that rapeseed breeders are facing and the basic goal of premature breeding is to meet the requirements of cultivation system in the south of China. Growth of existing rape varieties with long, mature late, in the rice-rice-oil triple cropping, the cultivation of rapeseed harvest in time of conflict and harvesting of rice cultivation, which is caused by rice fields, especially in winter idle Rice Field One of the main. Therefore, the development of paddy fields to rape, rape in particular. Rice Field, Rice Field growing need to address the larger issue of rape contradictions season, breeding early maturing varieties is the fundamental way to solve this problem, and appropriate to achieve this goal early flowering Basis. So on rape flowering habit and its molecular mechanism of imperative. SDG8 is a gene encodes a homologue of the yeast SET2 histone methyltransferase. When SDG8 loss of function in Arabidopsis it will make it a phenomenon of early flowering. SDG8 homologous genes in oilseed rape has been systematically studied, obtained the following results:1,Through sequence homology analysis, we cloned the full length cDNA, BraSDG8 in Brassica rapa for the first time.2,Through the Bioinformatics analysis, we found that the BraSDG8 and SDG8 are all belong to ASH1 family. The full-length BraSDG8 was 4963bp, which encoded 1654 amino acid residues. It included a AWS domain, a SET domain and a Post SET domain from 867 to 1060 consecutive amino acid. Arabidopsis's SDG8 gene contains the same functional active site. BraSDG8 and SDG8 belong to a family of ASH1 SET domain subfamily.3,A RNA was constructed interference vector which is expressed consitutively by 35S promoter and make the SDG8 gene's expression silencing in rapeseed or Arabidopsis.4,The RNA interference vector was transferred into Brassica napus No. XY15 and Arabidopsis thaliana (Columbia) by Agrobacterium-mediated transformation.One positive transformation of seedling was detected in Arabidopsis. Seven positive transformations of seedlings were detected in rapeseed. These paved the way for further study on the function of BraSDG8 gene in rapeseed.