Effects Of Water Logging Stress On Physiological Features And Differential Expression Of Protein Of Sesamum Indicum Seeding

The purpose of this study was exploring the physiology and biochemistry response and adaptation mechanisms of sesamum seedling under waterlogging stress. A pot of this study simulated flooding conditions, the flooding gradient of sesame seedling stress. Water logging and control test set (not to waterlogged) treatments with the water treatment facilities Od, Id,3d,5d,7d played a total of five water time (intensity) gradient. Yuzhi 11 varieties were used as the experimental material waterlogging type. Physiological and biochemical response, mainly through the determination of sesame leaf relative water content, MDA and proline content in leaves, three protective enzymes (SOD, POD, CAT) activity and other indicators reflect. Mechanism by side trying to flooding Od 7d and two samples of the whole protein extraction of sesame leaf cells, through the two-dimensional electrophoresis of the sesame leaves of two treatments for specific protein expression analysis to reveal the difference.The results are as follows:The result revealed that among the sesame seedling waterlogging at different times,, after flooding sesame showed significant morphological changes compared with the control. At the beginning, growth of aerial parts becomes slow, leaves began to slump and turn white, thick petioles, and the part of the root under the soil becomes slender, and became black. The water content of the leaves decreased compared with the control.In the flooded treatment, sesame leaves MDA showed a significant upward trend, the MDA content was positively correlated with stress time; as flooding stress time increases, the leaf proline content first increased and then decreased indicating that sesame seeds make their own resistance to waterlogging stress response to cope with stress, but then a sharp decline in proline content, and sesame seeds themselves may be the mechanism of anti-damage related Stress. After the flooded treatment of three protective enzymes (superoxide dismutase SOD, CAT, catalase and peroxidase POD) increased first and then decreased. And, SOD and CAT in the flooding peak and then decline after 5d, POD peak and then decline in the 3d. The control activity showed a slight increase steadily increasing trend. Using two-dimensional electrophoresis technology, the differential analysis of the waterlogged 7d and control (non-flooded treatment) sesame seedling leaves showed that flooding increased significantly after 7d protein spots of 5, significantly down 20 points; 7d waterlogging and does not deal with the characteristics of the expression of 42 proteins. The results of the research made a firm foundation in promoting differential protein sequence and function.