The Effect Of Moderately Halophilic Bacteria On Soil Physical And Chemical Properties Of Cotton, Enzyme Activity And Microbial Diversity

Object:In this paper, a comparative analysis method, applied different concentrations of moderately A6 halophilic bacteria in cotton soil physical and chemical properties, microbial diversity, soil enzyme activity were studied. A6 moderately halophilic bacteria of cotton to soil physical and chemical properties, microbial diversity, soil enzyme activities, as modified by soil microorganisms to lay a theoretical foundation.Methods:(1) The need to train A6 moderately halophilic bacteria. (2) Inject into the cotton fields during the cotton planting in soil with different concentrations of bacilli A6 moderately halophilic bacteria. (3) By alkaline hydrolysis-hydrolytic nitrogen diffusion method, determination of available phosphorus molybdenum blue method, potassium dichromate oxidation-organic matter outside the heating method, the use of pHs-3c model pH meter determination of pH. (4) Coating culture microbial by dilution plate count. (5) Apply with 3,5-dinitrosalicylic acid colorimetry invertase, phenol-Determination of urease sodium hypochlorite, copper Determination of protease.Results:(1). Halophilic bacteria applied the same concentration of physical and chemical properties of soil compared to that in the early emergence stage applyed bacteria had three halophilic bacteria to soil hydrolyzable nitrogen content at a high level, are significantly higher than halophilic bacteria had CK applyed hydrolysis of soil nitrogen content, indicating that halophilic bacteria applied to the soil of nitrogen content on soil hydrolase significantly enhance the effect. II concentration of bacteria applied water for the hydrolysis of the nitrogen content of soil change is flat to down compared to the concentration of water for the facilities II on soil bacteria to enhance the role of the hydrolysis of the nitrogen content is relatively stable, especially in the flowering and boll and boll opening of the performance of obvious.Overall, the appropriate concentration II levels that applied bacteria soil hydrolyzable N content increased a certain role. CK bacteria are not available phosphorus content of the soil showed a growing trend, and other facilities over the soil fungus to increase available phosphorus did not change significantly, but only in the cotton boll opening reflects the facilities of the bacteria on the increase in soil available phosphorus content facilitating role. From the seedling stage to the budding stage, to the boll opening period imposed by halophilic bacteriaⅠ,Ⅱ,Ⅲsoil potassium as a whole showed an increasing trend, except during the blooming period other than the seedling stage, bud stage, boll opening strains of the facilities hadⅠ,Ⅱ,Ⅲsoil concentrations are higher than ever halophilic bacteria CK soil available potassium. Halophilic bacteria that is applied to the whole soil of cotton has improved significantly the role of potassium. Shi had bacteriaⅠ,Ⅱ,Ⅲof soil organic matter content is higher than in the cotton seedling stage halophilic bacteria have not applied the CK soil organic matter content, indicating that halophilic bacteria applied to cotton to increase soil organic matter content promoted only in the cotton the emergence stage. In the cotton budding and flowering and boll of cotton through the soil bacteria appliedⅠ,Ⅱ,Ⅲwere lower than the soil pH had halophilic bacteria CK soil pH, halophilic bacteria at this stage shows the cotton soil The role of pH has decreased.(2). In the bud stage, during the blooming period and boll opening stage, is not applied the number of bacteria are lower than CK soil bacteria strainsⅠ,Ⅱ,Ⅲsoil bacteria. In the flower and boll opening and boll stage of the bacteria showed no CK soil actinomycetes bacteria are lower than facilitiesⅠ,Ⅱ,Ⅲsoil actinomycetes. In the seedling stage, bud stage, flowering and boll and boll opening of the four periods of the applied strain is not generally show the soil fungi are lower than CK bacteriaⅠ,Ⅱ,Ⅲsoil fungi. This trend shows that in the cotton A6 moderately halophilic bacteria exert beneficial to increase the number of soil microorganisms to increase the number and diversity of soil microorganisms. The microbial activity of soil enzymes, or produce some secondary metabolites, so increase in the number of soil microorganisms to promote plant growth.(3). Sucrase out only in the cotton seedling and budding showed high activity, and with the applied strain increased with increasing concentration, CK<Ⅰ<Ⅱ<Ⅲ. A6 moderately halophilic bacteria exert beneficial activity of soil improvement. From the seedling stage to boll opening of cotton on the whole, urease activity in all treatments was not significant. Moderately halophilic bacteria that imposed on the A6 in soil urease activity were not obvious. Soil protease and urease showed the opposite trend, in the cotton bud and boll stage of the processing of land at a lower level of soil protease, and the difference between the various treatments was not significant, but in the cotton boll opening of harvest season each treatment had a higher protease activity in the soil of land, III highest soil protease activity, indicating that A6 moderately halophilic bacteria applied in the soil to enhance the protease activity plays a role.(4). Microbial diversity and soil enzymes there is a certain correlation:the number of bacteria with the protease was significantly negatively correlated; actinomycetes and fungi were significantly negatively correlated; actinomycetes and invertase activity was significantly negatively correlated. Soil enzyme activity is good or bad soil quality reflects an important aspect of soil enzyme activity can reveal the status and trends of soil development, soil fertility and can be used to evaluate the effect of soil improvement measures, to improve the agro-ecological environment and promote ecological agriculture development of scientific basis.Conclusion:This study found that moderate A6 halophilic bacterium cotton applied for microbial diversity of view, reasons for the increase caused by soil microbes may be due to moderate halophilic bacterium A6 imposed changes in the structure caused by soil. Changes in soil structure will change the activity of soil enzymes, soil properties also changed, and ultimately affect the soil nutrient cycle and nutrient uptake of cotton to cotton growth and development have an impact..