Expression Analysis Of The Alternate Oxydase AOX1 In Physiological Male Sterile Induced By Chemical Hybridizing Agent SQ-1

wheat is the second mainly crop in our country, and the application of the heterosis is the major approach to increase the outcome of wheat. There are three approaches used for heterosis of wheat, cytoplasmic male sterility, Photo-thermoperiod Sensitive Genic Male Sterility and physiological male sterility induced by chemical hybridizing agent SQ-1. Of which the physiological male sterility induced by chemical hybridizing agent is a crucial and convenient way to utilize the heterosis, and the advantage of which is established the threshold of breeding on the latest achievement. By the advantages of complete effect for pollen killing, wide scope of utility and no negative Impact on other organ of wheat,Chemical hybridizing agent SQ-1 is one of the most excellent male gametocide in the world. There was a big breakthrough for the mechanism of the male sterile both from the protein level and the molecule level in recently, but for the specific mechanism action is remains to be research. Therefore, we used the flag leaf and anther of genotypic male sterility ms(Kots)-90-110, physiological male sterility induced by chemical hybridizing agent SQ-1near isogenic line of ms(Kots)-90-110 backcrossed 5 generations and inbred once, BC₅F₁ and its near isogenic line BC₅F₁ (fertility restored by rk5451) as the materials, according to the NCBI published sequence of gene AOX1αand AOX1c (family of Alternate Oxydase AOX1), followed by the real time primer designation in light of its conservation district, and the expression analysis of real time PCR in the uninucleate anther stage, binucleate anther stage and trinucleate anther stage, thereby revealed the relation of male sterile process and the expression character of the alternate oxydase AOX1. Therefore, provided theoretical basis to the physiological male sterility, the results as follows:1. analyzed the expression of AOX1αat the uninucleate anther stage, binucleate anther stage and trinucleate anther stage in the genetical male sterility, physiological male sterility and the normal fertility via the real time PCR, as the flag leaf and the anther as experimental subjects, the result shows, the express discipline of two sterility lines was correspond with the fertility and appears a low expression in flag leaf, the highest in the uninucleate anther, a decrease in binucleate and trinucleate anther stage. For the physiological male sterility, it exhibit a similar discipline expect a slight increase in the trinucleate anther stage. Compared with the genetical sterile and the fertility in the same stage, the expression of AOX1αwas increased and decreased significantly in the uninucleate and binucleate anther stage respectively. Therefore, we inferred that the ectopic expression of AOX1αin both uninucleate and binucleate anther stage was impacted on the pathway of cyanide-resistant respiration, thus result in the abortion of anthers.2. analyzed the expression character of AOX1c of the three materials and their different organ(flag leaf and anther) in different stage as presented above via real time PCR, shown that the expression discipline was correspondence in the three materials at the each stage, all of them exhibited a increasing followed by a decrease. While for the amount of the expression, there was no significant differential in physiological male sterility and the normal fertility line, but the expression at unincleae anther stage in genetic male sterility was obviously lower than physiological male sterility and the normal fertility. Therefore,we inferred that the AOX1c was not participating in the regulation of physiological male sterility.3.According to the result of the real time PCR, we found that both the genes AOX1αand AOX1c take part in the metabolic regulation of genetic male sterility.but AOX1c didn,t take part in the metabolic regulation of physiological male sterility, it Indicate that male sterility mechanism of physiological male sterile and genetic male sterile may be different.