Effect Of Different Diets And Feeding Frenquency On The Feeding, Growth, Muscle Composition And Digestive Enzyme Activities Of Juvenile Elopichthys Bambusa

A 56d feeding experiment was conducted to study the effects of four culture model (Fl:feeding forage fish with 1 time every day; F2:feeding forage fish with 2 times every day; A1:feeding artificial diets with 1 time every day; A2:feeding artificial diets with 2 times every day) on feeding, growth performance, muscle composition and digestive enzyme activities of the juvenile Elopichthys bambusa. The results showed that:1. Effect of different diets and feeding frenquency on the feeding of juvenile Elopichthys bambusaThe feeding ratio of fish feeding with forage fish is higher than fish feeding with artificial diets, and the 2 time/d groups have higher feeding ratio than 1 time/d. The order of dry weight feeding ratio is:F1<A1<F2<A2, the diet has significantly influence on dry weight feeding rato, but feeding frequency has no significantly influence on dry weight feeding rato.2. Effect of different diets and feeding frenquency on the food utilizing of juvenile Elopichthys bambusaThe order of food coefficient is:A1<A2<F2<F1. The diet has significantly influence on food coefficient, the food coefficient of fish feeding with forage fish is higher than fish feeding with artificial diets. The order of dry weight food coefficient is: F1>A1>F2>A2. The 1 time/d groups have higher dry weight food coefficient than 2 time/d, the dry weight food coefficient of fish feeding with forage fish is higher than fish feeding with artificial diets. The dry weight food coefficient of F1 is significantly higher than the other groups.The order of protein conversion rate is:F1<F2<A1<A1. The diet has significantly influence on protein conversion rate, the protein conversion rate of fish feeding with forage fish is lower than fish feeding with artificial diets, feeding frequency has no significantly influence on protein conversion ratio.3. Effect of different diets and feeding frenquency on the growth of juvenile Elopichthys bambusaThe order of growthrate is:A2>F2>A1>F1. The weight growth of F1, F2 and Al is increasing during 0-56d, the weight growth of A2 is increasing during 0-42d, but decreasing during 43-56d. The SGR of each group has significantly difference during 0d-14d and 14d-28d, A2>F2>A1>F1. F2 and A2 have no significantly difference during 28d-42d, the SGR of F1 and F2 are significantly higher than A1 and A2.A2 has the maximum final body weight, F1 is the minimum. Compared to the forage fish, with the same feeding frequency, fish feeding with artificial diets was significantly higher in fianl weight, SGR, growth ratio (P<0.01).Under the same diets, with the increasing of feeding frequency, the growth performances and suvival were significantly increased (P<0.01), but tht feeding frequency has no significantly different on condition factor (P>0.05). The diet and feeding frequency have no interaction to the indexes(P>0.05).4. Effect of different diets and feeding frenquency on the muscle composition of juvenile Elopichthys bambusaThe content of crude protein, fat of F2 are significantly higher than the other groups. Compared to the forage fish, with the same feeding frequency, fish feeding with artificial diets was significantly lower in the content of crude fat (P<0.01), but is not significantly different on crude protein and ash.Under the same diets, with the increasing of feeding frequency, the content of crude protein, fat were significantly increased (P<0.01), but content of moisture is significantly decreased. Different diets and feeding frequency have interaction on FC, the content of crude protein and lipid (P<0.05), but no interaction on the other performances (P>0.05).5. Effect of different diets and feeding frenquency on the digestive enzyme activities of juvenile Elopichthys bambusaThe activities of protease in the intestine were significantlyly higher than in the hepatopancreas (P<0.05). The activities of protease in the intestine of fish feeding with forage fish is higher than fish feeding with artificial diet, F1 is significantly higher than F2, A2 is significantly higher than A1. The activities of protease in the hepatopancreas of F2 is significantly higher than the other groups.The activities of lipase in the intestine were significantlyly higher than in the hepatopancreas (P<0.05). The activities of lipase in the intestine of F1 is significantly higher than the other groups(P<0.05). The activities of lipase in the hepatopancreas of A1 is significantly higher than the other groups(P<0.05).The activities of amylase of Fl, F2, and A2 in the intestine were significantlyly higher than in the hepatopancreas (P<0.05). The activities of amylase in the intestine of fish feeding with forage fish is higher than fish feeding with artificial diet (P<0.05). The activities of amylase in the hepatopancreas has no significant difference in the groups(P>0.05).According to the dry weight feeding rato, dry weight food coefficient, protein conversion rate, growthrates and muscle composition, the better combination is the A2 group.