The Bioresource-chemistry And Antioxidation Of Pyracantha Fortuneana

Pyracantha fortuneana, a kind of abundant wild plant resource, is usd as both food and medicine. It is not only rich in nutrients, but also contains abundant isoflavonoid components with lots of health maintenance efficacy, such as excellent whitening effect, antiatheroscloresis, increasing immunity and facilitating digestion. But some bottlenecks, for example, few study in the resources chemistry of P. fortuneana, lacking in scientifically effectively methods about component analysis and bioassay evaluation, resulted in its products lacking in resource characteristics and its industrialization has not been a noticeable trend. In this paper, we analysed the bioresource chemistry constituent of P. fortuneana, and studied their dynamic change law and vitro antioxidant activity. This would promote the industrialization development and efficient utilization of P. fortuneana. Some results were obtained as follows:1. We established serial analytical methods about P. fortuneana bioresource chemistry compounds.Established the fractional analysis method of multiple isoflavonoid components by spectrophotometry; Established the overall evaluation method of group characteristics of phytochemical compounds using HPLC finger-print; Established the activity-evaluation method of P. fortuneana extracts by vitro antioxidant activity.This method could be used to study the components and activity of bioresource chemistry compounds, which supplied an efficient method for the attributional analysis and quality monitoring of P. fortuneana and its products.2. We researched the characteristics of bioresource chemical components of P. fortuneana systematically. After analysis of LC-MS results and comparison with published reports, some major common peaks were identified as chlorogenic acid, dicatechin, glucoside, galactose, rutin, hyperoside and isohyperoside;The antioxidant activity of P. fortuneana extracts were better than BHT obviously. It can be used as the raw material for functional products, cosmetic and botanical drug.3. We monitored the dynamic changes of bioresource chemical components of P. fortuneana and found that their contents are different during ripening period. This provide theoretical basis for the comprehensive utilization of P. fortuneana and the determination of optimal harvesting time.4. We optimized the extraction process of antioxidant components of P. fortuneana: ethanol concentration 71%, pH 3.2 and extracting temperature 51℃. The extract was more stable in acid condition and low temperature evades under light, adding some flavonoid compounds such as chlorogenic acid and tea polyphenol could increase the stability, which indicated that there were intermolecular co-pigmentation and synergistic effect between its internal flavonoid and anthocyanin components.