Lipid Microdmains On Plasme Membrane And Response Of Plant Lipid Microdomains To Pathogen Infection

Lipid microdomain related to pathogen and host interaction. To study Lipid microdomain structure and function help us understanding the interaction between plants and pathogens.We used a well used cell line-3T3-L1preadipocytes from mice to study the lipid micro-structural protein in the cellular localization, interaction of the lipid micro-proteomics and lipid micro-protein Cavin-1biological functions; we also studied Arabidopsis thaliana, to explore changes of lipid microdomains in the process of plant pathologen infection. The results were follows.1. We used immunoprecipitation and protein mass spectrum to analyse protein of Caveolae. The resluts indicated that some novel protein, such as, Kanadaptin, Storkhead box2, Eukaryotic translation elongation factor1, Mediator of DNA damage checkpoint protein1, Titin and Septin appeared in Caveolae fragments with Caveolin-1.2. We also anaylzed the interaction between Caveolin-1and other lipid raft protein Cavin-1, Flot-1and Aqp-1. The resluts revealed that there were some slight interaction between Caveolin-1and Cavin-1, flot-1and aqp-1peotein. This suggested that Caveolae and non-caveolae lipid raft may occur some transformation.3. Biochemical experiment and over-expression of Cavin-1fused to EGFP protein in cos7cell revealed that Cavin-1could form insoluble protein sedimented in vivo and in vitro especially under circumstance of gathering of Caveolin-1or over-expressing Cavin-1for long time. This nature of Cavin-1might have some biological function in forming of Caveolae.4. Detergent, temperature or deprivation of protein function could affect the interaction of between Cavin-1and lipid microdomain.We builded new model to illustrate a new way for biogenesis of Caveolae. There were two ways for biogenesis of Caveolae. The one was a de nove pathway that cluster Caveolin-1recruited free Cavin-1to aggregate in together, and then form Caveolae, finally transfered Caveolae to plasma membrane. The alterative way of Caveolae biogenesis was that Caveolae could bidirectional form or deform into non-Caveolae lipid raft and Caveolae.5. Cellular plasma membrane of Arabidopsis thaliana was treated by spore of Botrytis cinerea. The lipid raft on treated plant showed an increasing in protein content, altering of proteome, but no evident changed in distribution of sterol-a lipid raft marker on plasma membrane. These suggested that grey mould treat may change the structure and lead to some biophysical and biochemical alteration in lipid raft on Arabidopsis plasma membrane.