The Effects Of Nutrition Level On The Growth And Morphology Of Forestomach Epithelial

Two experiments were carried out in the present study 1 Effects of nutrition level on the growth and development of foerstomach 2 Effects of sodium butyrate infusion on morphology of forestomach epithelium.1 The effects of nutrition level on the growth and morphology of forestomach epitheliumEight healthy growing goats were selected and divided into two groups (n=4), high nutrition level (HL) and low nutrition level (LL). Goats in both groups were fed with peanut straw at ad libitum. In addition, goats in HL group were fed with 300 g concentrate, divided into four times a day (75 g each time). Water was given ad libitum. The experiment lasted for 6 weeks. Body weight of the goats was recorded once every week before feeding. At the end of experiment goats were slaughtered and the rumen and omasum were weighed with and without contents. Rumen and omasum epithelial tissue samples were taken and preserved into 4% paraformaldehyde for morphological studies.Average daily gain in body weight was significantly (P<0.01) higher in the HL than LL. No difference between two groups was found in the full rumen and omasum weights but the empty rumen and omasum weights were significantly (P<0.05) higher in the HL than LL. However, the length and surface area of rumen epithelial papillae were significantly (P<0.05) higher in HL than that of LL, but no statistical significance was found in the width and the number of papillae per cm2 between two groups. Histological slices of rumen epithelium analyzed by Image-Pro Plus 6.0, showed that the number of cells increased significantly (P<0.05) in the SG layer and (P<0.01) in the SG and SS layers. However, nutrition level has no significant effect on the omasal epithelial layer but the number of cells in SB and SG-SS layers showed an increasing tendency (P> 0.05) and (P=0.078) respectively, in LL group. It was concluded that diet with high nutrition level could promote the growth of rumen epithelium where as peanut straw intake favors the omasum growth. 2 The effects of infusion of sodium butyrate on the morphology of forestomach epitheliumSeven growing goats of approximately same body weight and age, fixed with rumen fistulae, were divided into two, the control (n=3) and treatment (n=4) groups. Goats were fed 200g concentrate two times a day (100 g each time) and hay ad libitum. Water was freely available for 24 hours. The treatment group was infused intraruminally with a final concentration of 0.3 g/kg buffered sodium butyrate maintained at pH 6.8 and the control group was infused with the same volume of PBS. The treatment lasted for 2 weeks. At the end of experiment goats were slaughtered and the rumen and omasum epithelial tissues were taken in 4% formaldehyde for histological studies.Color of rumen epithelium was darker in treatment group but no difference was found in color between tow groups. The dimensions (length and width) and surface area of rumen epithelial papillae were significantly higher, (P<0.05) and (P<0.01) respectively, in treatment than that of control group. The density (number of papillae per cm2) was not significantly (P=0.994) different between the groups. Histological slices analyzed by Image-Pro Plus 6.0, showed that the amount of corneous layer in the rumen of the treatment group was significantly (P<0.01) higher than that of the control group, while the amount of SG and SS layer were significantly (P<0.01) higher than that of the control group, and the number of the SB, SG and SS in average unit were also significantly (P<0.01) higher than that of the control group. The amounts of SG and SS layer on omasum were significantly (P<0.05) lower than that of the control. In contrast, the layer of stratum corneum was fewer than the control (P=0.139), but no significance was found. The amounts of cells in SG and SS per area unit on omasum were significantly lower than the control group (P<0.05), and the amounts of cells in SG and SS per length unit on omasum were lower than the control group(P=0.065). It was concluded that infusion of sodium butyrate could promote the length and width and surface area of epithelia papilla in rumen as well as the counts of epithelium SG and SS;meanwhile,the layer of SC in a normal phyloical range. infusion of sodium butyrate has no effect on the layer of SC of omasum,but the amount of SB as well as SG and SS have decrease tendency.