Proteomic Research Of Rice Plasma Membrane Protein Under Blast Stress

Blast, caused by the filamentous ascomycete Mangnaporthe oryzae (Hebert) Barr,is one of the three most devastating of rice diseases, and Serious impact on rice yield and quality. when the Mangnaporthe oryzae infect the Epidermal cells of rice, both the bulbous invasive hyphae and the extensional hyphae are outside of the host cells. they all surrounded by a plant-derived extra-invasive hyphae membrane(EIHM).Plant plasma membrane protein is one of the unique structure of the protein. Features embeded in the membrane of cells so that this class of protein sin the junction area with the outside world. Mediated signal transduction between the outside world and perform many basic and important cell biological functions, also plays an important role in plant defense responses against pathogen infection through take part in the signal transduction pathway.In order to study role of the plasma membrane proteins in the process of the rice blast resistance. In this paper, we used an improved membrane protein extraction method and two-dimensional electrophoresis method to analyze the differences of the plasma membrane proteins expression, which extract from the leaves of the Heikezijing that a Japonica rice variety from Taihu Lake region. By comparing before and 24 hours after inoculation,we found that 28 proteins expression was different.which 20 were up-regulated expression and 8 were down-regulated expression.28 protein spots with predicted functions in plant defense were identified by MS and MS/MS. All of the induced proteins can be divided in to five types:first associate with metabolizing, such as ribulose-1,5-bisphosphate carboxylase/oxygenaselarge (RuBisCO),Y putative senescence associated protein; second, associate with signal transduction, such as annexin and glutathione-S-transferase (GST); third,associate with regulating peptide,such as ACC oxidase (ACO),Developmentaly regulated plasma membrane polypeptide (DREPP);fourth, associate with detoxification enzymes, such as Ascorbate peroxidase (APX),Tyrosine kinase (PTKs); fifth, hypothetical protein, such as 14-3-3 like protein. Considering the MS identification results and previous studies, we cloned a remorin protein from the rice and do some research for its function. Previous studies showed that remorin protein is a plant specific protein. It is take part in Arabidopsis, tobacco and other plants in the biotic stress and abiotic stress process extensively. In leguminous and solanaceous plants are involved in plant disease resistance, But now for the specific resistance mechanism is still unclear. By through analysis the impression level of the remorin protein in resistant and susceptible varieties between different time segment.We found that the expression of OsREM1.2 inresistant and susceptible varieties are obvious differences. The expression in the resistant varieties was significantly higher than the expression level of susceptible. These means OsREM1.2 protein may play an important role in rice blast resistance.