The Preliminary Study On SEA Brine In Inhibition Of Pathogenic Fingi And Application

In this paper,we studied the sea brine in inhibition of pathogenic fungi and explored its potential application, mainly on its properties and composition, its inhibition on pathogenic fungi of plant, its salt injuries effects on plant, its influences on defense enzymes and its inhibition on pathogenic fungi growing in corn leaf . The results are as follows :1. The Baume degree of sea brine was 32.8°Bé, and the content of salt was 44.1%. The main anions in sea brine were Cl^-, Br^- and SO₄^2- , and the concentration was 204.00 g/L, 3.56 g/L and 74.20 g/L, respectively. Cl^- was significantly highest than the other anions. PO43- was not detected , which was less than 0.30 g/L; The main cations were Na⁺, K⁺, Ca²⁺ and Mg²⁺. Amount of Mg²⁺ were highes(t83.00 g/L),and the content of Na⁺ and K⁺ was 28.50 g/L and 19.30 g/L, respectively; Amount of Ca²⁺ was less than 0.50 g/L.The main organics were alkanes, olefines, alcohols, esters, ketones, ethers and acid anhydrides; Additionally, the sea brine contains trace elements including B, Mn, Fe and Zn.The concentration was 0.240 g/L, 0.040 g/L, 0.068 g/L, 0.044 g/L, respectively.2. The sea brine could inhibit the growth of Alternaria solani, Alternaria alternata, Fusarium graminearum and Glorosprium musarum at concentration of 0.0050%. The inhibitory rate was 47.4%, 33.3%, 31.2%, 29.2% in turn. However, had no marked inhibiting effect on Rhizoctonia solani.3. We studied the effects of different concentration of sea brine on seeds germination , seedlings growth, stem length, natural weight, dried weigh of seedlings, and also studied its effects on the content of MDA , PRO and conductivity rate of leaves. These tests proved that the lower concentration of sea brine(0.05% and 0.10%) had no salt injuries on tomato seedlings. On the contray, it had promoting effect on its growth.4. A Study of the effects on defense enzymes in tomato and corn leafs reveals that 0.050% sea brine could significantly enhanced the activities of PAL, CAT and PPO in tomato and corn leaves. It suggests that sea brine possessing certain systemic acquired resistance in plants.5. The sea brine had inhibiting effect on the Fusarium graminearum growing in corn leafat concentration of 0.005% and 0.0050%, and the former(44.3%) is better than the later(28.3%).