| Test one: unrefined bean oil oxidated at home-made device with oxidizing conditions, Sampling at different time 0d, 7d, 14d, 16d, 18d and determining Acid value(AV),Peroxide value(POV),Iodine value(IV), Thiobarbituric acid reactive substances (TBARS) and fat acid. Result: 18d compare with 0d , AV, POV, TBARS significantly increased, respectively 1.7 times, 29.0 times, 19.0 times, but IV significantly decrease to 0.91 times(p<0.05). UFA significantly decreased from 84.0% to 82.2% (p<0.05). 18d compare with 16d, POV, TBARS, SFA and UFA have no significant difference(p>0.05). Conclusion: bean oil oxidated at home-made device, the oxidation temperature is 80℃, POV and TBARS can be used as indicator of rapid thermal oxidation. When the bean oil been oxidated to 18days, the rate slows down.Test two: There are two types of the fat in forage of grass carp, one is oxidation of soybean oil(POV:128.7mmol O/kg,TBARS:24.8 ug MDA/g), other is normal oil(POV:7.2mmol O/kg,TBARS:0.7ug MDA/g). In the types four different levels were set (2%, 4%, 6% and 8%), total of 8 groups. The initial weight of 35.9±2.1g for the study of grass carp are feed in cages of cement pool in the indoor for 12 weeks. We collected serum and hepatopancreas at 2, 4, 6, 8, 10, 12 week and measured biochemical indicators of hepatopancreas and serum and did biopsy of hepatopancreas; we also determinated growth parameters at 12week.Result:①The SGR of control group (CG) are larger than corresponds to the SGR of experimental group (EG), and the FCR have the opposite trend. Mean of SGR by CG 20.4% larger than the mean of EG. With levels of oil increased, growth of EG slowed. With levels of oil increased, growth of CG slowed.②EG and CG (≥4%) hepatopanceas were damaged by cholangiole deposition and fatty liver, then hepatopancreas of CG (≥4%) were fibrosis and hepatopancreas of EG were necrosis. With levels of oil increased and culture time increased, these lesions increased.③In the capacity of antioxidant of serum, little difference from week mean of EG SOD and CG. With levels of oil increased, mean of EG and CG SOD have down. Week mean of EG MDA were by 28.5% larger than CG. With levels of oil increased, week mean of EG MDA up, week mean of CG MDA down.④Indicators of hepatic function, week mean of AST and CHE in serum of EG were separate by 8.7% and 4.1% smaller than CG. With levels of oil increased and culture time increased, week mean of AST and CHE in serum of EG have the trend of first down then up, week mean of AST in serum of CG up and CHE of CG down. Mean of AST in hepatopanceas of EG were by 24.0% smaller than CG.⑤In the capacity of antioxidant in hepatopanceas, mean of SOD in hepatopanceas of EG in 12 week were little difference with CG, its MDA were by 18.1% smaller than CG. With levels of oil increased, SOD in hepatopanceas of EG have the trend of first up then down, MDA have the trend of first down then up. With levels of oil increased, SOD in hepatopanceas of CG have the trend of first down then up, MDA have the trend of first up then down.⑥Study by intestinal permeability, at 12 week, mean of intestinal MDA and serum DAO of EG were separately by 22.2% and 8.7% smaller than CG. With levels of oil increased, MDA in intestinal of EG have the trend of first down then up, DAO in serum have the trend of first up then down. With levels of oil increased, MDA in intestinal of CG have the trend of first down then up, DAO in serum have little change. Conclusion: In the four levels of oil by 2%, 4%, 6% and 8%, feed oxidized soybean oil to grass carp on growth and hepatopancreas have adversely affect and with levels of oil increased, this effect aggravated. In the four levels of oil by 2%, 4%, 6% and 8%, feed non-oxidized soybean oil (≥4%) to grass carp on growth and hepatopancreas have adversely affect and with levels of oil increased, this effect aggravated too.Test three: Set oil level was 2%, feed were added normal oil and oxidated oil, and then added silymarin (666.7g/t), curcumin (1333.3g/t), yeast DV (750g/t) for tentative observating three drugs on the protection of grass carp feeding oxidized soybean oil. The same research methods with test two.Result:①SG and CuG had no effect on growth, then SGR of yeast DV were by 16.0% larger than OG, and FCR were by 38.1% smaller than OG.②Three drugs had a certain inhibition by cholangiole deposition and fatty liver.③Week mean of SOD and MDA in serum of CuG and YG were smaller than OG, but little difference with SG.④Week mean of AST and CHE in serum of three drug groups were smaller than OG. AST in hepatopanceas of SG and CuG were larger than OG, but YG were little difference with OG.⑤SOD and MDA in hepatopanceas of SG have little difference with OG. SOD in hepatopanceas of CuG and YG were larger than OG, but its MDA were smaller than OG.⑥Intestinal MDA and serum DAO of SG have little difference with OG. Intestinal MDA of YG has smaller than OG and serum DAO has little difference with OG. Intestinal MDA and serum DAO of YG have smaller than OG.Conclusion: Silymarin (666.7g/t), curcumin (1333.3g/t) and yeast DV(750g/t) have the positive impact to the hepatopancreas of grass carp which were feed by oxidation of soybean oil, but optimal addition of three drugs remains to research. |
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