Filamentous Fungi And Ectomycorrhizal Fungi Coupling Relationship, The Korean Pine Litter Decomposition

Both ecto-mycorrhiza fungi and filamentous fungus are common in forest system, meanwhile, the arbuscular mycorrhizae is a symbioticsystem composed by ecto-mycorrhiza fungi cooperating with plant roots in soil. Pinus koraiensis exporting great amount of litter falls are signaficant mycorrhizae dependent species in northest arbors. The litter returns nutritious elements to plants through the degerations by microbiology among which fungi is the most pivotal disintegrator and act as the key link in forest nutritious cycling. This article mainly researches the coupling relations and functional mechanisms of diverse fungi in the process of pinus koraiensis litter degerations.Using traditional methods, 12 types of filamentous fungi including foregone 8 and unknown 4 types were separated from Pinus koraiensis litter falls of original Pinus koraiensis forest in Wu Ying national nature park. It is acquired 12 kinds of large scale ecto-mycorrhiza fungal carposporophyte by stepping on spot surveies and sampling actions.Selecting high frequency filamentous fungi (Alternaria sp.) and ecto-mycorrhiza fungi (Xerocomus Pulverulentus and Clitocybe maxima) as materials commixed as diverse combinations, we investigated the ability of the different combination of strains to degrade fresh Pinus koraiensis litter from the mass loss rate, enzymes activity and the content of N, P during fermentation process. These results indicated that the total mass loss rate of AX (Alternaria sp. and X. Pulverulentus) was the highest to 18.33% in 60 days, while that of AC (Alternaria sp. and C. maxima) was just 13.27% because of antagonism. In 20 days the highest laccase activity of X. Pulverulentus, C. maxima and AX is 0.093U·mL^-1, 0.063U·mL^-1 and 0.047U·mL^-1, respectively. And during the same period the highest cellulase activity of Alternaria sp., AC and AX is 0.59U·mL^-1, 0.57U·mL^-1 and 0.53U·mL^-1, however, cellulase activity of AX is up to 0.48U·mL^-1 again in 50 days. During the fermentation process N content of AX reduced by 12.21% significantly, and P content is to 25.82% obviously higher than others combinations. Furthermore, the results of AC and AX keep coincidenced with the change of enzymes activity.It is showed that the mechanisms of different functional fungal groups to degrade forest litter and the interactions between fungal communities for further. Inhibitory actions or promotive effects among diverse fungi compose the fundamental step of forest litter degradation and nutritional cycling. The researches of these relations provide theory evidences and technology supports to the realization of"pluralism of ecto-mycorrhizal fungi as agent, RD of combinational biological fertilizers and hierarchy of application system"besides reasonable management of Pinus koraiensis forests. The perspective of application is optimistical.