Expression And Regulation Of HSP Genes In Silkworm, Bombyx Mori

Silkworm is an important economic insects and model organisms. Heat shock protein (heat shock protein, HSP) exists in biology widely, not only in the high expression under stress, but also widespread under normal circumstances. Ecdysone can promote the synthesis of nucleic acids and proteins, affecting carbohydrate lipid metabolism, affecting the central nervous system and immune regulation. Rutin is a common flavonol of flavonoids can inhibit cell proliferation and induce apoptosis. In this paper, we analysised BmHSPs with the method of bioinformatics, investigated the transcription levels of BmHSP genes in Bombyx mori with dual-spike-in qPCR method. The main results are as follows:1 bioinformatics analysis of heat shock proteinsUsing bioinformatics methods analyzed heat shock proteins, the results showed that: Bmhsp20.4, Bmhsp20.8 and Bmhsp19.9 have the closest relationship, small heat shock proteins were highly homologous, and Bmhsp21.4 was an independent branch in evolution. The secondary structure analysis of small heat shock proteins revealed that the sequences of 7 silkworm small heat shock proteins were quite conservative containing the similarα-helix region. Predicting the binding sites of transcription fators on promoters of BmHSPs revealed that the binding sites were different but had similarities, suggesting that maybe they were associated with fuctions,2 the expressions of BmHSPs in tisusses inducted by ecdysoneExpressions were determined in the midgut, fat body and malpighian tubules after induction by ecdysone. The results showed that: we could not detect the expression of Bmhsp70. In midgut, expression of Bmhsp21.4 increased at 2h after induction implied its importance. In fat body, transcription of Bmhsp25.4 kept in a high level. The expressions of genes were different in tusseus and timing,3 the expressions of BmHSPs in tisusses inducted by rutinWe detected the expression of each gene in tissues with qRT-PCR. The results showed that: the expression of Bmhsp70 was not detected; transcriptions were very different of genes, and different genes showed different expression patterns.We inferred the promoter binding sites may have some correlation with gene expressions.In this study we analysised ten heat shock protein genes with bioinformatics method, illustrate the expression levels of BmHSPs in different organizations inducted by ecdysone and rutin, we found that different gene has different expression modols after different induction in tissues and time, and the differences in gene expression may be related to the binding positions of transcription factors.The results can provide data for studying the function of BmHSPs in vivo and for subsequent research.