Energy Type Beet Varieties Screening And Identification Techniques

The energy problem is threatening the economic development of countries and affecting the living of human beings nowadays. In order to solve the energy problem, all countries in the world have carried out research on fuel ethanol. The production of fuel ethanol by microbiological fermentation becomes a focus topic in the field of research and exploitation of fuel ethanol around the world. Energy beet, which has wide planting area, simple fermentation process and high translation rate of alcohol, is a promising raw material used to produce fuel ethanol. In recent years, studies of energy beet have sprung up, however, no comprehensive identification technology of economic parameters about energy beet. In this study, different beet lines were screened by optimizing the fermentation condition of saccharomyces cerevisiae strain 1373. And the identification technology of economic parameters about energy beet was concluded.Six parts were searched in this paper. The first part was the field experiments. The second part was the screening of the most suitable strains. Translation rates of ethanol of four strains were compared, and then the strain that had the highest ratio of ethanol was screened for the further experiment. In the third part, the medium of fermentation were optimized. The optimum content of nitrogen and different inorganic salt of ethanol fermentation was obtained from the four factor and three levels of orthogonal fitting. The fourth part was mainly about the optimization of fermentation condition, including fermentation temperature, fermentation initial pH and inoculation amount. In the fifth part, the energy beets were screened, and the economic parameters and the identification techniques were identified. The translation rates of ethanol from different beet lines were studied, in order to identify the energy beet lines which produced the highest translation rates of ethanol. In the sixth part, 10L fermentation test was made.Firstly, based on the field experiment, the translation rates of ethanol of four strains were compared, the saccharomyces cerevisiae strain 1373 was screened by fermentation test, whose translation rate of ethanol is 72.16%. So the saccharomyces cerevisiae strain 1373 was used to do the following tests.Secondly, the optimum composition of the medium was: NH₄SO₄0.5%, CaCl₂ 0.5%, KH₂PO₄1.0%, MgSO₄0.5%. The optimum fermentation conditions were: fermentation temperature 32℃, fermentation initial pH 5.5 and inoculation amount 10%.The translation rates of ethanol from different beet lines were studied using the optimized fermentation conditions above. The identification technology was concluded: saccharomyces cerevisiae strain 1373 under the condition of fermentation temperature 32℃, fermentation initial pH 5.5 and inoculation amount 10%. The content of nutrients in fermentation medium was NH₄SO₄0.5%, CaCl₂0.5%, KH₂PO₄1.0%, MgSO₄0.5%. Based on the above condition the fermentation test was made. Ethanol translation rate was used as a criterion to distinguish energy beets. Three commercial lines of energy beets were screened: TYEB03, TYEB06 and zhongtian207/09.Finally, based on the optimized fermentation medium and conditions, fermentation experiment was carried out in 10L bioreactor. The results of fermentation test in bioreactor are superior to the results in laboratory.