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Effects Of Simulated Drought Stress With Polyethylene Glycol On The Seed Germination And Cutting Of Jute And Kenaf

Posted on:2012-01-03Degree:MasterType:Thesis
Country:ChinaCandidate:L YangFull Text:PDF
GTID:2213330371958101Subject:Crop Genetics and Breeding
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Drought, salt, low temperature (chilling injury) are three non-biological factors affecting crop growth and restricting crop production. The loss caused by drought on crops in all non-biological stress accounting for first place. Fiber crops is the fifth-largest group after grain, cotton, oil and food crops, and is extremely unique economic crops in our country. Among them, jute and kenaf are recognized the dominant crops in the 21st century, they are very versatile. Jute fiber quality is better than kenaf, but its drought resistance is less than kenaf that limited jute production severely. Kenaf itself has some drought resistance, but has not yet to makes a comprehensive and systematic drought-resistant resources appraisal, and no any reports on water stress of kenaf cutting. So making a comprehensive and systematic drought-resistant resources appraisal system and breeding the high drought resistance jute and kenaf varieties is very important to develop the jute and kenaf production.This research is divided into two parts:First, take 6 copies of Corchorus.capsularis (38,No.3,No.4,09-10,09-30,09-35),5 copies of Corchorus.olitorius(09-1,09-2,09-40,09-41,09-43) a total of 11 jute germplasm resources, and 4 kenaf germplasm(P3A,R4,R10,R11) as materials, research effects of different PEG concentrates on germination index of jute and kenaf seeds. In order to find a evaluation system of evaluating drought resistance in seed germination of jute and kenaf, and choose the stronger drought resistance varieties in germination, to provide the reference for the other growth periods of drought resistance identification. Second, take 4 jute materials (09 zhan 16,09 zhan 17,Zhongyin No.1 of jute,Zhong No.1 of jute) of the cuttings and 4 kenaf materials(P3A,992, R10, R11, R12) of the cutting as materials, research effects of different PEG concentrates on cuttings of jute and kenaf. In order to research effect of PEG simulated drought stress concentrates on cuttings of jute and kenaf, gradually establish evaluation system of drought resistance identification of jute and kenaf cuttings, choose the stronger drought resistance varieties in jute and kenaf cutting. The main results obtained are as follows: 1. The germination energy, germination rate, germination index, vigor index, buds fresh weight, radicle long and germ long etc. of all the tested materials is decrease when the PEG concentration increase. In addition to the correlation of radicle length of P3A,R4 and RIO between PEG concentration are not significantly enough, the other indexes and the correlation between the concentration of PEG were all significantly, and can be used for seed germination of jute and kenaf evaluation of drought resistance. The vigor index and buds fresh weight have the best correlation results with PEG concentration.2. The rang of the drought-tolerrance in all materials seeds:Round 09-30> Round 09-35> Round 38> Round 09-10> Round 4> Round 3; Long 09-41> Long 09-40>long 09-43> Long 09-2> Long 09-1; R11> R10> P3A> R4. The drought resistance of Corchorus.capsularis in drought resistance of seed that Round 09-30 tested the best, Round 3 is worst relatively. The Long 09-41 is the best and Long 09-1 is the worst in Corchorus.olitorius. R11 is relatively good, R4 is relatively bad in kenaf.5% to 10% of the PEG concentration can be chose as a suitable concentration to distinguish differences of drought resistance between Corchorus.olitorius; 15% to 20% of the PEG concentration to distinguish the difference of drought resistance between Corchorus.capsularis made the ideal of the concentration; 10% to 20% of the PEG concentration is suggested to distinguish the difference of drought resistance of kenaf.3. A certain concentration of PEG significantly inhibited the rooting of jute and kenaf cuttings.15% and above PEG concentration have a strong inhibitory effect on the rooting of jute and kenaf, not even the normal survive. The kenaf cuttings were dead before rooting. Kenaf cuttings root more slowly than jute cuttings about 8 days.4. The correlation between the soluble protein content of jute and kenaf cuttings with the concentration of PEG are complex. The soluble sugar content is positively correlated with PEG concentration. Root vigor, root length, root surface area and root number are all significantly negatively correlated with PEG concentration. Root volume of the correlation between the concentration of PEG addition to Zhongyin No.1 of jute was not significant, the rest of the materials to reach 1% under all the difference. Therefore, the soluble protein is not suitable for evaluating the drought-tolerance of cuttings. The remaining six indicators can be used as evaluation of the cuttings of kenaf and jute drought resistance indicators.5. Drought resistance of jute cuttings:09 zhan 16> 09 zhan 17> Zhong No.1 of jute> Zhongyin No.1 of jute; drought resistance of kenaf cuttings:992> R10> P3A> R11.
Keywords/Search Tags:jute, kenaf, polyethylene glycol, seed germination, cutting, drought stress
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