Corylus Screening Of Endophytic Fungi In Plant Functional Types

In this study, Corylus was presented as the research subject to isolate andidentificate endophytic fungi with biological activity. The activity assay of the crudeextract was tested and preliminarily purified and fermentation medium was optimized inorder to improve its biological activity.Eight endophytic fungi were isolated from the roots of hazelnut. By themorphological identification, a preliminary identification was stated as two strains ofPenicillium, a strain of Fusarium, a strain of Neurospora, a strain of Gibberella, a strainof Cladosporium, a strain of Hypocrea and a strain of Paraphaeosphaeria. Theendophytic fungi were cultured and their biological activities were tested. A strain withantioxidant activity, called JTLR-1, identified as Penicillium sp, was screened throughthe consolidated total antioxidant capacity determination of the results of the DPPHclearance and FRAP value indicators.The antioxidant activity of JTLR-1was analyzed by six kinds of antioxidantcapacity tests which were the scavenging DPPH of the JTLR-1fermentation brothextract, its hydroxyl radicals, the ability of oxygen free radicals, its total antioxidantcapacity and restore capabilities. And Vc, BHT were served as controls. JTLR-1fermentation broth extracts on showed clearance to the DPPH, Hydroxyl radicals,oxygen free radicals in some degree, and showed total antioxidant capacity andreducing capacity. The antioxidant capacity of JTLR-1fermentation broth extractshowed a certain dose-effect relationship with its concentration, which could bedescribed as that with the concentrations increasing, the antioxidant activity improvedin correlation. Compared with the control BHT and Vc, the hydroxyl radical scavengingactivity, reducing power, total antioxidant capacity to scavenge oxygen free radicalsability to inhibit the peroxidation inhibition of β-carotene/linoleic acid system of thesample were higher than those of BHT and lower than those of Vc; and scavengingDPPH was similar with that of Vc and BHT. The total phenolic content of JTLR-1fermentation broth extract was1.852%, and the total flavonoids content was1.957%.Correlation studies showed that the antioxidant capacity of the JTLR-1fermentationbroth extract, total flavonoids and total phenols had the significant correlation.And the good correlation between the different antioxidant methods was also shown.。By the column chromatography of silica gel, effective substances in the JTLR-1fermenting liquor extract could be roughly separated with impurities, and two groups ofhigh antioxidant capacity fractions were obtained, one named fraction5(ethyl acetate:ethanol=5:2), and the other named fraction6(ethyl acetate: ethanol=5:1). DPPHradical scavenging test showed that IC50of the clearance rate of the purified fraction5was up to1.05±0.11mg/mL, and the fractions6was up to0.99±0.09mg/mL.This study explored the impact of nutrients on oxidative substances. In order toachieve the optimum fermentation conditions of antioxidants, by comprehensive use ofsingle factor experiment, PB experimental design and RMS method, an antioxidantactive ingredient suitable for JTLR-1was achieved. The medium included: Glucose30g/L, Peptone3g/L, Nitrate copper0.318mg/L, Zinc sulfate0.631g/L, Ferrous sulfate0.1864mg/L. And the IC50value of the antioxidant capacity was0.8101mg/L in thiscondition.