Cloud Beer X Large Grain Wheat F <sub> 2 </ Sub> Groups Grain Functional Components Of The Genetic And Qtl Analysis

Barley is mainly used for feed, human consumption, and most importantly raw materials of beer. Resistance of barley is strong and rich in germplasm resources, so its functional components has great potential for development and utilization. Studying on functional components of Barley is conducive on development of barley functional food, at the same time it is in favor of foundation prepared for the prevention of diseases of the daily diet. However, there were little evaluation of germplasm resources and genetic studies about barley medicinal and health functional components alkaloids and resistant starch. Therefore, it is very necessary to focus on the evaluation and genetic analysis of functional components in barley grain with barley genetic basis and background studies. In this paper, using F2population for the material, analyze the composition of its kernel function, establish high and low gene pool, carry out genetic analyze of barley grain and preliminary QTL analysis, The results show that:1. In this study, the colorimetric determination of barley grain functional ingredients, research shows that functional ingredients have a good linear relationship with absorbance, the correlation coefficient is between0.9976-0.9993. It is highly repetitive to meet the test requirements. Therefore, using the UV-visible spectrophotometric colorimetric method to measure barley grain functional ingredients is feasible, simple, fast and high-volume detection.2. Total alkaloids and the resistant starch content of the F2population have large variation, the average content of total alkaloids is8.005mg/100g, the amplitude is0.64-42.99mg/100g, as the maximum content of varieties is70times of the minimum content of varieties. The average content of RS is1.848%, the amplitude is0.42-7.79%, as the maximum content is19times of the minimum content. This shows that the resistant starch content of genetic have large variation. Alkaloids and resistant starch content differences can be used for genetic analysis.3. The absolute value of kurtosis and skewness that plant height, ear length, the average grain number per ear, grain length, grain width agronomic traitsthe in F2population is less than1.96, fit the normal distribution. The F2population have a full separation in plant height, ear length, the average grain number per ear, grain length, grain width.4. In36traits from five traits and four functional components of the171groups in the F2generation, nine pairs of traits achieve at highly significant or significant correlation, they are total flavonoids and alkaloids, total flavonoids and GABA, GABA and grain length, GABA and grain width, plant height and spike length, plant height and total grains per panicle, grain width and grain length, grain length and spike length, spike length and total grains per panicle. The number of them whose orrelation coefficient greater than0.3is two, they are grain width and grain length, spike length and total grains per panicle, and achieving significant. GABA and grain length, GABA and grain width achieve significant positive correlation.5. The composite interval mapping analysis of the agricultural traits, RS (qRSl), alkaloids (qALl)and total grains (qGNSl)of F2,three QTLs related to the barley grain RS, alkaloid and total grains content were detected. They located between chromosome1GBM1336-Bmac90, phenotypic variance explained by4.28%,3.66%and63.51%, respectively. This shows that the same or similar gene family impact RS, alkaloid and total grains of the barley grain.