Phosphoproteomic Study Of Rice (Oryza Sativa L.)Responses To Cadmium Stress

Rice (Oryza sativa L.) is one of the most crucial crops in our world, with the growth of population, enhancement of rice resistant to poisonous environment which caused by industrial development, is strategic important for us. Cadmium (Cd) is one of the most toxic heavy mentals, it can be easily transported and accumulated between plants, and will have bad affects on rice growth and development, as well as sharply reduce rice yield.By using proteomic methodology, which based on two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), phosphoproteins in rice leaves and phosphoproteins'differential expression pattern after Cd treatment have been studied and analised. This research is aims to further elucidate molecular mechanisms of rice responses to Cd stress, and provide theoretical support for breeding of Cd resistant rice varieties.Major achievements of this research is that, firstly, we have selected a sensitive line DH69and a Cd resistant line DH28from a DH population consisting of127DH lines, this DH population was established through anther culture of the Fl hybrid between rice varieties of ZYQ8and JX17; secondly, an effective protocol for phosphoproteins separation and enrichment form leaves and also for2D-PAGE was established, which can result in a high resolution ratio of separated phosphoprotein spots, providing a new way for quantitative phosphproteomic study; thirdly,53discrepantly expressed phosphoproteins in leaves of two lines were identified, they are belong to seven function groups. In DH28which is more tolerant to Cd sress, about49%of its variant phosphoproteins participate in metabolic process in rice, while the proportion is30%in DH69. Other phosphoproteins related to incellular signal transduction, regulation factors of gene expression, trasporter and membrane fusing.