Coining And Analysis Of HSP70and HSP90of Quadrastichus Erythrinae Kim (Hymenoptera: Eulophidae)

Erythrinae gall wasp (EGW) Quadrastichus erythrinae Kim, as a quarantine pestinsect to China, belongs to Quadrastichus of Tetrastichinae (Hymenoptera,Eulophidae). Since first found in2003it has spread rapidly to Pacific islands oftropical and subtropical, margins of Asia and North America, and caused severedamage to Erythrinae plants. This paper researched on the HSP70and HSP90ofQ. erythrinae, cloned their the full length cDNA, analyzed nucleotide sequences andamino acid sequences by bioinformatics. On the basis of the above, we tested themRNA expression levels of HSP70and HSP90in adults under high temperature stress,and discussed their thermotolerance mechanism. The results were shown as follows:1. The full length cDNA of Q. erythrinae HSP70(GenBank: JN971028) is2468bp, containing a1932bp open reading frame, a72bp5`-untraslate region, a464bp3`-untraslate region. Encoding644amino acid (GenBank: AFC76151) with apredicted molecular mass of71.1161kDa.2. The full length cDNA of Q. erythrinae HSP90(GenBank: JN971029) is2922bp, containing a2163bp open reading frame, a107bp5`-untraslate region, a622bp3`-untraslate region. Encoding721amino acid (GenBank: AFC76152) with apredicted molecular mass of83.3323kDa.3. Q. erythrinae HSP70and HSP90are both hydrophilic proteins, mainly locatedin the cytoplasm. Q. erythrinae HSP70contains three signature sequences of70kDaheat shock family, ATP/GTP-binding site, peptide-binding domain,Actin-like proteinATPase domain, the non-organellar consensus motif of eukaryote, and EEVDsignature sequence. Q. erythrinae HSP90contains five signature sequences of90kDaheat shock family, Histidine kinase-like ATPase domain in N-site and MEEVDsignature sequence on C-site.4. Semi-quantitative RT-PCR results showed that under the treatment tempatures,mRNA expression levels of Q. erythrinae HSP70and HSP90showed the trend ofrising first and then drops roughly.1h heat shock treatment at37℃, mRNAexpression levels of Q. erythrinae HSP70reached maximum, for2.77times of the control group.1h heat shock treatment at39℃, mRNA expression levels of Q.erythrinae HSP90reached maximum, for3.44times of the control group.