| Silkworm(Bombyx mori) is an important model and economic Lepidoptera insert, hasalways been concerned about the origin and evolution. It is the theoretical basis of thesilkworm genetics and breeding. Silkworm have a variety of ecological types and systemsgroups, such as the Chinese system, Japanese system, Tropical system and European system.Phylogenetic relationships among silkworm breeds and genetic distance of silkworm origin,evolution, germplasm identification, preservation and use of varieties of silkworm parentselection practice, has important theoretical significance and practical. The genetic diversityof53silkworm (Bombyx mori.) varieties conserved in Shaanxi province was analyzed byAFLP(amplified fragment length polymorphism)method in order to utilize germplasmresources in silkworm breeding and provides evidences for researches on the selectiveimproved variety of silkworm from Northwest area in China.1Methods of DNA extraction from diapause of silkworm eggsExtraction of DNA from silkworm is the basic work of molecular genetic researchtechnology, and also is the most common problems of identification and purity testing. In thispaper, by comparing the result of three methods on DNA extraction, we found a better wayand DNA extracted from a single eggs could meet the requirement for basic molecularresearch.2Genetic diversity of silkwormTotal535bands were amplified by14pairs of primers combinations, and472(88.22%)of which showed polymorphisms.3Genetic Distance and Cluster AnalysisCalculate the genetic distance between silkworm varieties by NTSYS-pc2.1. the genetic distances of53varieties are between0.1934-0.3491. The cluster analysis of53silkwormvarieties and the each system by UPGMA and NJ. Study subjects were divided into threegroups, the Japanese line and European line have the closet genetic distance than Chinese line,four monovoltine trimolter varisties origined from Northwest area in China belonged to aseparate group by Cluster Analysis using UPGMA. |
PDF Full Text Request