Response Of Moso Bamboo To Phosphorus Treatment And Research Of PheWRKY2

Moso Bamboo(Phyllostachys edulis) is the major bamboo species for shoot and timberuse in China. It has long been the most important economic bamboo in China, for its highproduction and wide distribution. However, its growth is constrained by a variety ofenvironmental conditions. The soil in natural distribution of moso bamboo is usuallyphosphorus deficiency, and low phosphorus stress is one of the limitations to its productivity. Itis so significant to explore the potential strains those are not sensitive to low phosphorus stress,and improve yield, resistance and quality of moso bamboo using genetic engineering.In order to clarity the physiological responses which forces to the low phosphorus stressand the function of WRKY transcription factors in moso bamboo, the following works havebeen done in the study. The effects of the different phosphorus concentration on growth ofmoso bamboo were studied under hydroponic conditions. With the decreasing phosphorusconcentration, root length and root surface area was increased. The dry biomass and root/shootratio of moso bamboo tended to rise and then fall as the phosphorus concentration increased.When the concentration of phosphorus was1mmol·L-1, the dry biomass is the biggest. Thecontents of IAA, ZR and GA3in root have diversity change along with different concentrations.The contents of IAA, GA3and ABA in leaves were decreased in each concentration, while ZRwere increased. Different phosphorus concentration had significant effect on the contents ofendogenous hormone in moso bamboo, and then had influenced growth of roots of mosobamboo.The WRKY transcription factors exsit only in plants and members of them are widelyimplicated in defenses responses and various other physiological processes. A PheWRKY2genewas cloned by RT-PCR in the study. PheWRKY2encodes a peptide harboring one WRKYdomain and one zinc finger, falling into subgroup of WRKY proteins. The protein localizes inthe nucleus, might has the function of transcription regulation. The expression characteristics and transcription activate activity of PheWRKY2were studied in the research. Overexpressionvector for PheWRKY2was constructed and transformed into Arabidopsis thaliana, and thelow-phosphorous tolerance of transgenic plants was studied. By the yeast one hybrid technique,the protein encoded by PheWRKY2exhibited the function of transcription activation in yeast.Transgenic plant advanced in flowering. They had lower sensitivity to low phosphorusconditions and could enhanced their utilization of organic phosphorus. As low phosphorusrelated gene, the research of PheWRKY2can provide a theoretical basis to screen out strainswith low phosphorus tolerance and potential applications to improve the production of mosobamboo.