Effects Of Cordyceps Sinensis And Tripterygium Wilfordii Polyglycosidium On The Podocytes In Rats With Diabetic Nephropathy

Objective To investigate the effects of cordyceps sinensis (CS) and Tripterygium Wilfordii Polyglycosidium(TWP)on the podocytes of diabetes nephropathy (DN) rats.Methods Rat models of DN were established and they were divided randomly into normal control group(Group A),DN group (Group B),CS group (Group C), TWP group(Group D). After 12 weeks, the urinary protein quality in 24h, Glu, BUN and SCR were measured. The pathological changes of Kidney organization were observed after HE staining. Using the TEM we measured the minuteness structural changes of podocyte.Use indirect immunofluorescence histochemistry method to detect protein expression of the nephridial cortical Nephrin and Podocin.Results 1.Comparing to the group A , the degrees of the SCR, BUN were significant higher (P<0.05); and the GLU,KW/BW and UAER in 24h were also obviously higher (P<0.01) in group B,C,D . and there were phenomenon of glomerula r disorder, chronic tubulointerstitiul damage and glomerular podocyte lesion; The protein expressions of Nephrin and Podocin in nephridial tissue were lower in group B,C,D,. 2. Comparing to the group B, the KW/BW and UAER were lower(P<0.01), in group C,D. The degrees of the GLU, SCR, BUN were also lower(P < 0.05); The pathomorphological improvements were exhibited in glomerulus, tubules and podocytes in group C and D;.The protein expressions of Nephrin and Podocin were higher in nephridial tissue in group C and D.Conclusion CS and TWP have protective effect on podocyte of diabetic nephropathy rats,The mechanisms may involve the up-regulative expressions of Nephrin and Podocin, and the improvement of podocyte lesions.