The Study Of Probucol As Antioxidant In The Rat Brain

Ojective:The study was carried out to prove Protctive effects of Probucol for normal and cerebral Ischemia-reperfusion injuries(CIRI) rats on anti-oxidation and to find out the effective mechanism by testing changes of the content of 12/15 lipoxygenase,protein carbonyl,nitroprotein and LPO in the brain tissues of normal rats or with CIRI.Method:male SD rats, The weight between 280g-310g. All focal CIRI (60min, 24hr) models in rats were made by line bolt method. Pro bucol is dissolved by the 0.5% CMC suspension. Intragastric administration of medications by gastric tube every day. Experiment consisted of two parts. The fir st part: Probucol effect on normal old rats. Normal old rats were given probucol (500mg/kg,1 week,2 weeks, 3 weeks) intervention. Comparison of 12/15 lipoxygenase, prot -ein carb onyl, nitro protein, lipid peroxide changes. PartⅡ:Effect of probucol on cerebral ischemia-reperfusion injury in rats.Divided into two parts.1:The first production model of cerebral ischemia-reperfusion injury, given after prob -ucol (100mg/kg, 500mg/kg,800mg/kg,1 week,2 weeks). Comparison of the detection of index changes.2:Be given probucol (100mg/kg,500mg/kg,800mg/kg,1 week,2 weeks,3 weeks,4 weeks), post-production model of cerebral ischem ia-reperfusion injury. Comparison of changes in the detection index. Western-blotting detection of 12/15-lipoxygenase, levels of nitro- protein, Through detection of MDA to inspect LPO. Detection of protein carbonyl through spectrophotometer.Result:1:in normal rats after cerebral ischemia-reperfusion injury,12/15 lipoxygenase, protein carbonyl, nitro protein, lipid peroxides were significantly higher than normal, CMC can not be given alone to reduce the detection index.2: Normal old rats given Probucol 500mg/kg, reduced the indicators, have the same time-dependence.2 weeks were significantly different(P<0.05).3:pre-treated with probucol group compared with ischemia-reperfusion group:Before cerebral ischemia treated with different doses, different time pretreatment process Probucol, All Can inhibit the 12/15 lipoxygenase, protein carbonyl, nitro protein, lipid peroxidation induced by brain ischemia reperfusion injury,.moreover in a dose-dependent, With the enhanced role of increasing concentrations of probucol.2 weeks time-dose effect of the various dose groups has more effective than 1 week time-dose, There is a Stati stically significant (P<0.05).4 weeks of continuous application of 500mg/kg of which gradually increased inhibition.4: injury before probucol treatment group compared with ischemia-reperfusion group, After cerebral ischemia treated with different doses, different- process time Probucol can inhibit the rise of 12/15 lipo xygenase, protein carbonyl, nitro protein, lipid peroxide induced by cerebral isch emia and reperfusion injury.2 weeks of each dose group was stronger than 1 week in each group, the difference was significant (P<0.05).5Compare the groups(100mg/kg,500mg/kg,800mg/kg) which ischemia after the drug treatment with the groups which ischemic before ischemia treatment, the effect of each dose group is significant (P<0.05).Conclusion:1:Probucol has a protective effect on stress in normal old rats and cerebral ischemia and reperfusion injury induced oxidative stress, And were correlated with the dose and duration.2:Probucol mechanism against oxidative stress is multifaceted,and relate with its structure scavenging activity of phenolic hydroxyl oxygen.