| Objective: To observe health bone with osteoarthritis of rabbit knee articular cartilage morphology and histological changes in cartilage cell secretion of matrix metalloproteinase -1 (MMP-1), matrix metalloproteinase -3 (MMP-3), specific matrix metalloproteinase inhibitor (TIMP-1), interleukin-1β(IL-1β) and the role of chondrocyte apoptosis index (AI) and value-added index (PI) of the different effects; Health Treatment of Knee Bone Clinical efficacy of arthritis, to investigate the mechanism of traditional Chinese medicines and provide the basis for clinical treatment.Methods: Study: 35 New Zealand rabbits were randomly divided into 7 groups, normal control group, model control group, kidney group, blood circulation group, heat Lishi Group, chills wins wet group, kidney and blood detoxification group, the former two groups of saline, after the five groups were given ZuoguiWan Shixiaosan, four wonderful casual, Herba Tong pills, bone health side. Gross observation after 8 weeks the general performance of joint structure, the morphological changes observed under light microscope and to detect cartilage and synovial membrane in the MMP-1, MMP-3, TIMP-1, IL-1βexpression in positive cases, MMP-3, TIMP -1 average gray detection of chondrocyte apoptosis index and proliferation index. Clinical observation: 60 patients with knee osteoarthritis were randomly divided into test group and control group, experimental group of 30 patients taking healthy bone side, the control group of 30 patients taking glucosamine sulfate, were treated for 6 months.Results: Study: morphological changes observed under light microscope: According to Mankin's score, optical microscopy of articular cartilage and synovial degeneration score, model control group scores were significantly higher than the control group (P <0.05), that modeling success; healthy bone side group Shixiaosan group, Herba Tong pill group, four wonderful powder group, scores were Zuoguiwan group compared with the control group were lower (P <0.05), showed that each group can be reduced more than score to improve cartilage lesions; Strengthening bone group scores were compared with other Chinese groups were lower (P <0.05), that group of healthy bone with cartilage lesions in the lower scores improved better than the other Chinese groups. Histological changes observed under light microscope: Zuoguiwan group Shixiaosan group, four wonderful powder group, Herba Tong pill group, the group Strengthening bone articular cartilage, synovial MMP-1, MMP-3, IL-1β-positive expression were lower than the model control group, bone health side were decreased more significantly; Zuoguiwan group Shixiaosan group, four wonderful powder group, Herba Tong pill group, the group health bone with articular cartilage, synovial TIMP-1 positive expression were higher than the model control group, bone health side group increased more significantly. Cartilage and synovial MMP-3 in the average gray areas: model control group and the normal control group mean increased (P <0.05), show that the model successfully; healthy bone side group Zuoguiwan group, four wonderful scattered groups Shixiaosan group, Herba Tong Wan group compared with the control group, respectively, the mean reduction (P <0.05), that the above groups by reducing MMP-3 in cartilage cells to improve cartilage lesions of the average gray; Strengthening bone group were compared with all the other reduce the number of Chinese groups (P <0.05), that group of healthy bone with cartilage cells in reducing MMP-3 is better than the other average gray Medicines. Cartilage and synovial TIMP-1 in the average gray areas: model control group and the normal control group mean increased (P <0.05), shows that successful modeling; healthy bone side group Zuoguiwan group, four wonderful scattered groups Shixiaosan group, Herba Tong Wan group compared with the control group mean increased (P <0.05), that the above groups can be increased TIMP-1 in cartilage cells to improve cartilage lesions; healthy bone with traditional Chinese medicine group and other groups mean increased (P <0.05), that group health bone with cartilage cells increased TIMP-1 is superior to the other average gray Medicines. Enhanced index of chondrocytes: the model group compared with the control group mean decreased (P <0.05), shows that successful modeling; Strengthening bone group and the model number of the control group were higher (P <0.05), showed that bone health party can add value by stimulating cartilage cells to improve cartilage lesions; other Chinese groups were compared with the control group mean increased, but the difference was not statistically significant (P> 0.05). Apoptotic index of chondrocytes: the model group compared with the control group mean increased (P <0.05), shows that successful modeling; healthy bone side group Shixiaosan group, Herba Tong Wan group compared with the control group mean were lower (P <0.05), that the above groups can reduce the apoptotic index of chondrocytes to improve cartilage lesions; four wonderful scattered groups Zuoguiwan group compared with the control group mean decreased, but the difference was not statistically significant ( p> 0.05); healthy bone with traditional Chinese medicine group were compared with the other numbers were lower (P <0.05), that group of healthy bone with the cartilage in the lower apoptotic index was superior to the other Chinese groups. Clinical observations: After treatment, TCM syndrome score and severity, the experimental group and control group, the difference was statistically significant (P <0.05), that the experimental group in improving symptoms and severity of traditional Chinese medicine is better than the control group; lower C reactive protein (CRP), the both groups, the difference was statistically significant (P <0.05), between the two groups, the difference was not statistically significant (P> 0.05), shows efficacy in reducing CRP is when the two groups.Conclution: The study showed that bone health can only improve the degeneration of cartilage and synovial level score, effectively reduce the articular cartilage of MMP-1, MMP-3, TIMP-1, IL-1βexpression and MMP-3 mean gray and reduce the apoptotic index of chondrocytes, cartilage cells increased TIMP-1 value-added index and the average gray to improve the cartilage lesions, the specific mechanism remains to be further explored; clinical observation showed that bone health side effects treatment of knee osteoarthritis sure, and can reduce CRP. |
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