Induction Of MMP-2 And MMP-9 Release From Human Dermal Fibrolasts By Thrombin And Inflammatory Factors

Background: The dermal fibroblasts are the most important cells in skin wound healing which produce collagen and matrix metalloproteases. Gelatinase (MMP-2 and MMP-9) degrade collagen type IV and extracellular matrix components and play an important role in skin wound healing. Our previous studies have suggested that thrombin activated human dermal fibroblast releasing MMP-9. However, thrombin with pro-inflammatory factors IL-1β/ TNF-αor IL-1β/ TNF-αcontribute to evoking MMP-9 release from human dermal fibroblasts are largely unclear.Objective: To investigate thrombin and inflammatory factors IL-1β/ TNF-αevoking MMP-9 release from human dermal fibroblasts.Methods: Thrombin 5u/ml, IL-1β5ng/ml, 10ng/ml and TNF-α5ng/ml, 10ng/ml were added jointly or individually to human dermal fibroblasts cultured primaryly in vitor. Pro-MMP-2/9 activity were detected using gelatin zymography treated at 2h, 6h and 24h time point.Results: (1)At different time point (2h, 6h and 24h), thrombin 5u/ml can induce pro-MMP-9 release significantly, but fail to induce pro-MMP-2 release. At 6h time point and 24h time point, IL-1β5ng/ml, 10ng/ml and TNF-α5ng/ml, 10ng/ml can induce pro-MMP-9 release significantly, but fail to induce pro-MMP-2 release. Thrombin, IL-1βor TNF-αinduced pro-MMP-9 release from HDFs in a time dependent manner at different time point following incubation. (2)At different point time (2h, 6h, 24h), thrombin 5u/ml with IL-1β10ng/ml can induce pro-MMP-9 release markedly (P<0.05). Thrombin 5u/ml with TNF-α10ng/ml together can reduce pro-MMP-9 release markedly (P<0.05) at 2h time point while induce pro-MMP-9 release markedly (P<0.05)at 6h time point and 24h time point. (3)Thrombin 5ng/ml and IL-1β10ng/ml, TNF-α10ng/ml together can decrease pro-MMP-9 activity from HDFs (P<0.05). IL-1β10ng/ml and TNF-α10ng/ml had little influence on releasing of pro-MMP-9 activity from HDFs (P>0.05).Conclusion: (1) Thrombin and inflammatory factors IL-1βor TNF-αcan voking pro-MMP-9 release from human dermal fibroblasts, but not pro-MMP-2. With prolonging of time of voking, thrombin, IL-1βor TNF-αinduced pro-MMP-9 release from HDFs in a time dependent manner at different time point following incubation. (2) Thrombin with IL-1βtogether, or thrombin with TNF-αtogether can induce pro-MMP-9 release from HDFs while thrombin with IL-1β, TNF-αtogether decrease pro-MMP-9 activity from HDFs. (3) IL-1βwith TNF-αtogether have little influence on releasing of pro-MMP-9 activity from HDFs.