| ObjectiveTo analyze the homocysteine levels in the folic acid and/or vitamin B12 supplied Hyperhomocysteinemia patientsMethods160 Hyperhomocysteinemia patients were chosen from first affiliated hospital of traditional Chinese medical college, involved in this clinical trial. There were 20 cases whose folic acid and vitamin B12 both lower than the normal level; there 60 cases whose folic acid level is lower than normal range and vitamin B12 among the normal range; there were 60 cases vitamin B12 lower than normal range and folic acid among the normal range; there were 20 cases vitamin B12 and folic acid among the normal range.Selected standard:patients qualified the diagnosis of cerebral infarction and Hyperhomocysteinemia, male,55-80 years old.Exclusion criteria:acute infection, sever cacotrophy, hypothyroid, hyperthyrea, hypohepatia, renal inadequacy, hematological system related disease, therioma, experienced the operation of OrganizationTransplantation,have taken vitamin complex in half year,a serie of drug that influence the serum level of homocysteine,like carbamazepine and dilantin sodium.Group classify:After getting the permission of patients and their family members, according to the level of folic acid and vitamin B12, classify the patients into four groups, A, B, C, and D. A group:20 cases whose folic acid and vitamin B12 both lower than the normal level, supplied with regular dose of vitamin B12 and folic acid.for two weeks, then withdraw the blood of patients to check the Hcy serum level; B group,60 cases totally, with vitamin B12 Lower than normal range and folic acid level within normal range.randomly divise 60 cases into two subgroup B1 and B2. There were 30 cases in B1 group, so is the B2 group. The two groups were treated with vitamin B12 or vitamin B12-folic acid complex seperately, and then check their Hey serum level after two weeks supply. In C group; there were 60 cases which are folic acid level lower than normal range and vitamin B12 level within normal range. 60 cases in C group were randomly divised into two subgroup C1 and C2.There were 30 cases in C1 group, so is the C2 group.They were treated with folic acid and vitamin B12-folic acid complex, seperately. Then check their Hcy serum level after vitamin supply.for two weeks.In D group, there were 20 cases whose levels of vitamin B12 and folic acid both in normal range. They were supplied with regular amount of vitamin B12 and folic acid. then check their Hcy serum level after vitamin complex supply.for two weeks.Vitamin B12 and Folic Acid Supplied Dosen:Folic Acid,5 mg/d, oral administration, once perday; Vitamin B12 500μg/d, oral administration, once perday; course of treatment:two weeks. Method of Detecting Hcy:enzymic method. Machine Model:Olympus auto-biochemic alanalyzer. Kits are brought from:Peking jiuqiang biotechnology limited company. Statistical analysis is done by the software of SPSS 16.0. To check the difference in tHcy values between pretherapy and post-treatment in every group, paired t- test is applied. To check the difference between pretherapy and post-treatment in groups, One-Samples T Test is applied.α=0.05.Results the pretherapy level of Hey in A group is (33.029±12.812)μmol/L, post-treatment level of Hey in A group is (22.919±7.388)μmol/L, there is statistically significant difference between the pretherapy level and post-treatment level in A group (t=6.548, P<0.001). the pretherapy level of Hey in B group is (32.004±14.094)μmol/L, post-treatment level of Hey in B group is(20.213±7.510)μmol/L, there is statistically significant difference between the pretherapy level and post-treatment level in B group(t=5.719, P<0.001). The decrease of B1 group from pretherapy to post-treatment [(10.417±6.301)μmol/L], comparing with B2 group's [(13.163±11.646)μmol/L], there is no statistically significant difference between B1 group and B2 group. (t=-1.136, P=0.261)The pretherapy level of Hey in C group is (32.604±15.320)μmol/L, post-treatment level of Hey in C group is(19.287±7.099)μmol/L, there is statistically significant difference between the pretherapy level and post-treatment level in C group(t=6.109, P<0.001). The decrease of C1 group from pretherapy to post-treatment [(14.697±13.516)μmol/L], comparing with C2 group's [(11.937±8.074)μmol/L], there is no statistically significant difference between C1 group and C2 group(t=0.960, P=0.342). the pretherapy level of Hey in D group is (30.309±13.350)μmol/L, post-treatment level of Hey in D group is(28.474±9.889)μmol/L, there is statistically significant difference between the pretherapy level and post-treatment level in D group (t=1.831, P=0.083)ConclusionsVia intaking vitamin B12, folic acid there could be lowering effect for the level of homocysteine which is aroused by vitamin B12 and/or folic acid deficiency. If there is only foic acid deficieny occurred, the results of lowering Hey level have no difference between supplied with foic acid and foic acid-Vitamin B12 complex.vice versa. If there is only Vitamin B12 deficieny occurred, the results of lowering Hey level have no difference between supplied with Vitamin B12 and foic acid-Vitamin B12 complex. |
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