Study On The Antidiabetic Active Constituents Of The Stems Of Gymnema Sylvestre

Purpose: To study the chemical constituents from the stems of Gymnema sylvestre(Retz.)Schult. The key is to confirm the efficacy of medicine part and elucidate the compounds structure which was isolated from the stems of Gymnema sylvestre.Method: Proceed to Extract different polarity parts to be certained active sites through nonenzymatic glycosylation inhibit reaction from the stems of Gymnema sylvestre. Eight compounds were isolated by the modern chromatography techniques (macroporous resin, ODS and HPLC, prep-HPLC etc) from the stems of Gymnema sylvestre. Their structures were elucidated by the spectroscopic analysis (1~HNMR, (13)~CNMR, APCI-MS,MALDI/TOF-MS) and chemical methods.Result: Eight compounds were isolated from the the stems of Gymnema sylvestre (Retz.) Schult. These compounds were identified as Conduritol A (â… ),Stigmasterol (â…¡), Lupeol(â…¢),Stigmasterol3-O-β-D-glucoside(â…£),The sodium of22-hydroxy-longisipinogenin3-O-β-D-glucuronopyranosyl-28-O-α-L-rhamnopynosid e(â…¤), The sodium of22-hydroxy-longisipinogenin3-O-β-D-glucopyranosyl-(1â†'3)-β-D-glucuronopyranosyl-28-O-α-L-rhamnopynoside(â…¥),Oleanlicacid3-O-β-D-glucopyranosyl(1â†'6)-β-D-glucopyranside(â…¦),16-22-dihydroxy-olean-12-ene-3-O-β-D-xylopyranosyl-(1â†'6)-β-D-glucopyranosyl-(1â†'6)-β-D-glucopyranoside (â…§).Conclusion: Compoundsâ…¢,â…¤,â…¥,â…¦, andâ…§were obtained from this plant for the first time. And Compoundsâ…¤,â…¥,â…¦, andâ…§were obtained from the active site which can be used for structure-activity relationship study.