The Effects Of Astragaloside Ⅳ On The Neurocyte And The Expression Of PBRs After Cerebral Ischemia-reperfusion Injury In Rats

Objective:To investigate the effects of Astragaloside IV on the neurocyte and the expression of PBRs after cerebral ischemia-reperfusion injury in rats. Methods: Altogether 90 male SD rats were randomly divided into sham-operation group, The model group and Astragaloside IVtreatment group(10 mg/kg,40 mg/kg and 100 mg/kg). The rats of intervention groups were treated with Astragaloside IV injection intraperitoneally in 30 minutes before operation. The middle cerebral artery ischemia/reperfusion (IR)rat model was established by a modified Longa occlusion method, we observed the expression level of PBRs in core and penumbral area by immunohistochemical double staining technique. Apoptosis was examined by using terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labelling (TUNEL) staining in all rats. Results:(1) Compared to IR group, the score decreased in 40 mg and 100 mg group (P<0.05), the infarction volume decreased in 40 mg and 100 mg group(P<0.01). Compared to 10 mg group, the score decreased in 40mg group (P<0.05), the infarction volume decreased in 40mg and 100 mg group (P<0.01). (2) Compared to sham-operation group, the number of the apoptotic neural cells increased significantly in model group (P<0.01). Compared to IR group, the apoptotic neural cells of penumbra diminished, the expression level of anti-neuronal nuclei (Neun) of penumbra increased in AST 40 mg group (P<0.01), the apoptotic neural cells of penumbra diminished, the expression level of anti-neuronal nuclei (Neun) of penumbra increased in AST 100 mg group (P<0.05). Compared to AST 10 mg group, the apoptotic neural cells of penumbra diminished, the expression level of anti-neuronal nuclei (Neun) of penumbra increased in AST 40 mg group (P<0.05). (3) Compared to sham-operation group, the expression of GFAP increased obviously in model group (P<0.01). Compared to IR group, The expression of GFAP of penumbra diminished in 40 mg and 100 mg group (P<0.01). Compared to 10 mg group, the expression of GFAP of penumbra diminished in 40 mg group (P<0.05). (4) Compared to sham-operation group, the expression level of OX42 increased in model group (P<0.01), Compared to IR and AST 10 mg group, The expression level of OX42 in penumbra diminished in 40mg group(P<0.01), the expression level of OX42 of penumbra diminished in 100 mg group (P<0.05). (5) Compared to sham-operation group, the expression level of PBRs increased(P<0.01). compared to IR group, the expression level of PBRs and microglia of expressing PBRs of penumbra diminished in 40 mg group (p<0.01), the expression level of PBRs and microglia of expressing PBRs of penumbra diminished in 100 mg group (P<0.05). Compared to 10 mg group, the expression level of PBRs and microglia of expressing PBRs of penumbra diminished in 40 mg group (P<0.05). Conclusion:Astragaloside IV can protect ischemia brain tissue by decreasing the apoptosis of neuron cells and inhibiting the activation of PBRs of microglia.