Research On Phosphory Choline (PC) Bionic Modified Medical Dacron Materials And Anticoagulant Property

Lecithin is the composition of cell outer membranes. It is mainly constituted by the phosphorylcholine(PC) group with equal positive and negative charge on the surface which has been recognized as a good biocompatibility. The biomimetic modification of poly(2-methacryloyloxyethyl phosphorylcholine, MPC) on the surface of biomedical materials can efficiently improve the biocompatibility of the materials, and enhance the anticoagulation of the materialsIn this research, MPC was synthesized according to the method proposed by Ishihara in 1990. Polyethylene terephalate (PET, Dacron) was used as the substrate. Poly(MPC) was grafted on PET surface adopting atom transfer radical polymerization(ATRP) followed by a series of chemical reaction. The surface chemical composition and surface property of PET were qualitatively and quantitatively characterized using Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy(XPS), water contact angle analysis, and so on. The blood compatibility and cell compatibility were investigated using in vitro platelet adhesion, the lactate dehydrogenase (LDH), activated partial thromboplastin time (APTT), fibrinogen deformation and endothelial cell adhesion test and in vivo implantation test.The results of FTIR and XPS proved that the MPC polymer was successfully grafted on the surface of PET. After the biomimetic modification with poly-MPC on PET, LDH and APTT experiments showed that the PET surface possessed good anticoagulation and efficiently prohibited platelets adhesion on its surface. At the same time, the surface of poly-MPC modified PET could efficiently restrain fibrinogen adsorption and denaturalization. The surface of poly-MPC modified PET surface was in favor of adhering and growth of endothelial cells.