Effect Of Lobaplatin On Apoptosis Of Breast Cancer MCF-7 Cells And Expression Of MTDH Gene

Objective: Breast cancer is one of the most common malignant tumor with high incidence which is harmful to women health. Since the metastasis is the main death cause, the inhibition of tumor invasion and metastasis is very important for breast cancer treatment. overexpression of MTDH is frequently observed in carcinomas of breast and is correlated with poor clinical outcomes. Recent studies have established a functional role of MTDH in several crucial aspects of tumor progression, including invasion, metastasis, evasion of apoptosis and chemoresistance. MTDH may become a potentially valuable target in cancer treatments.Cisplatin is the basic drug in advanced breast cancer with certain curative effect, severe kidney toxicity and gastrointestinal side effects limit its clinical application, so development of efficient and low toxicity of platinum agents has been the scholars's goal. Lobaplatin is the third generation of platinum derivatives. with complete cross-resistance to cisplatin and the strong anti-tumor activity, Lobaplatin has been initially demonstrated in an important role in cancer therapy. Breast carcinoma cell line MCF-7 was used as research subject in our experiment. MTT and flow-cytometry assays were adopted to examine the influence of lobaplatin on the proliferation and apoptosis of MCF-7 cells. RT-PCR assay was used to detect the effect of lobaplatin on MTDH mRNA expression. It would be helpful to provide experiment basis for lobaplatin in breast cancer chemotherapy.Methods:1. Investigate the effect of lobaplatin on the proliferation of the breast cancer cells by MTT assay.2. Folw cytometry was used to detect the effect of lobaplatin on the apoptosis of the breast cancer cells.3. RT-PCR assay was used to detect the effect of lobaplatin on MTDH mRNA expression.Results:1. Different concentrations of lobaplatin at the same time inhibition rate compared with the control group were statistically significant (P<0.05). the effect of the same concentration of lobaplatin on MCF-7 cells for 24,48,72 hours time inhibition rate compared with the control group were statistically significant (P<0.05). lobaplatin can inhibit MCF-7 breast cancer cell proliferation, inhibition rate increased with the increase of drug concentration, which showed a dose-dependent effect; inhibition rate increased with the increase of time, which showed a time-dependent effect.2. The apoptosis rates of MCF-7 cells treated with 5mg/L, 10mg/L and 15mg/L lobaplatin were 19.39±0.67%, 24.53±1.20%, and 65.02±0.78%, respectively, which showed an obvious dose-dependent effect. Statistical significance was found between the lobaplatin-treated and the control group (P<0.05).3. The expression of MTDH mRNA could be inhibited by a certain concentration Lobaplatin and no more MTDH mRNA could be detected with more than 10mg/L Lobaplatin.Conclusion:1.Lobaplatin can inhibit the proliferation of MCF-7 cells obviously. which showed a dose and time-dependence relation. lobaplatin can induce breast carcinom cells to apoptosis in a dose-dependent manner.2.Lobaplatin could inhibit the proliferation of MCF-7 cells and induce them to apoptosis. Lobaplatin maybe affect the migration and invasion of breast cancer cells by inhibiting MTDH gene expression.