Neuroprotective Effect Of Osthole And Mechanism Research

PartⅠosthole protects neuros and affects the expression of Homer1a in the mechanical scratch model of culture neuro in vitroObjective Through osthole intervention the mechanical scratch model of culture neurons in vitro, to observe the neuroprotective effect of osthole and the role of Homer1a. Methods To establish the scratch model of neurons, lactic dehydrogenase (LDH) assay was used to assay cytotoxicity changes after neuronal injury, the expresstion of caspase3 and - Homer1a at different time points were detected by western-blot. The neurons were saperated into shamp group (neurons without the treatment of scratch and osthole), cnotrol group (neurons with scratch only) and OST groups(0.05mM). 24 hours after neuros scratch, the expression of Caspase3 and Homer1a were detected by means of Western-Blot. Results All neurons showed multiform transparent cytoplasm with round or oblonga nucleuses and connected each other to form reticular structure; The results of protein analysis showed that osthole could reduce the elevated level of Caspase3 protein and increase the level of Homer1a induced by scratch injury. Conclusion Decreased expression of Caspase3 and increased expression of Homer1a after the treatment of osthole in the scratch model of neurons indicated that the osthole can prevent neurons from the injury and the Homer1a protein may play an important role in the process.PartⅡOsthole protects PC12 Cells against MPP+ Induced Neuro damageObjective To investigate the neuroprotective effect of osthole on 1-methyl-4-phenylpyridinium ion (MPP+) induced neurotoxicity in PC12 cells.Methods PC12 cells were treated with MPP+ 2 hours after treated with or without different concentrations of osthole(0.01,0.05,0.1mM). 24 hours later, MTT was used to assay the cell viability. Cytotoxicity was quantitatively assessed by measuring the activity of lactate dehydrogenase (LDH) released from the damaged cells into the culture medi um. The expression of Bax, Bcl-2, cytochro me c, caspase3 and Ho mer1a were detected by Western-blot. Results Pretreat ment with different concentrations of osthole on PC12 cells significantly reduced the loss of cell viability, the release of lactate dehydrogenase, the increase in Bax/ Bcl-2 ratio, the generation of and cytochro me C induced by MPP +. Pretreat ment with different concentrations of osthole could increase the Ho mer1a expression. Concl usion The results suggest that pretreat ment of osthole protects PC12 cells against MPP + induced cytotoxicity and the Ho mer1a protein may play an i mportant role in the process.