Spleen Yin Deficiency Syndrome Rats Motilin, Pepsin, Aquaporin-4 And Proteomic Changes In Experimental Research

Traditional Chinese Medical(TCM) science holds that spleen is acquired foundetion and origin of producing qi and blood.The obtainance of living substances mainly relies on spleen after birth. The spleen participates in the whole digestive syetem function as well as the endocrine system, autonomic system, immune system and metabolic blood system, combing with the modern medical. TCM consideres that the spleen Yin is the foundation of acquired Yin, which govers nourishing, dispersing the essence to the whole body and participates transformation and transportation and controlling blood. Recently, the academic more and more pay attention to discuss on serum, organs and proteomics technology of spleen Yin deficiency syndrome. This study is to establish the model of spleen Yin deficiency syndrome and explore the modern medical basis on occurrence and development of spleen Yin deficiency syndrome, comparing to control group.Purpose:This supplement of science evidence is to explore the mechanism of spleen Yin deficiency syndrome by observing the change of motilin (MTL),aquaporin4 (APQ4),pepsin and proteomics index of model rats, in order to reveal the essence of spleen Yin deficiency syndrome and treatment of spleen Yin deficiency syndrome with Chinese drugs.Materials and methods:Rats model of spleen Yin deficiency syndrome was established by the improper diet, overstrain and consumption of Yin fluid. These physiological conditions of each rat were observed after 16 days. The changes in stomach bottom tissue and ileum tissue of each rat were observed by microscope.The changes of expression in ileum tissue of APQ4 were detected by immunohistochemistry method. The changes of expression in serum of MTL were detected by enzyme-linked immunosorbent double antibody clip method (ELISA). The changes of expression in duodenal of activity of Pepsin by colorimetric method. The changes of expression in proteomics were idengtified between the contol and the experimental group by proteomics technology.Result:1. The result of MTL by ELISA:Compareing with control, the expression of MTL signicantly decreased (P<0.01) in experimental group and the TCM proof group Compareing with experimental group, the expression of MTL improved in TCM proof group (P<0.05)2. The result of Pepsin by colorimetric method:compareing with control group, the expression of Pepsin decreased signicantly (P<0.01) in spleen Yin deficiency experimental group, and the expression of Pepsin decreased (P<0.05) in TCM proof group. The expression of Pepsin had no any change between spleen Yin deficiency experimental group and spleen Yin deficiency TCM proof group.3.The result of APQ4 by immunohistochemistry:The expression of positive particles in the epithelial cells of the control is marked deeper brown and larger quantities by microscope. Compared with the control group, the expression of positive particles in spleen Yin deficiency experimental group is marked lighter brown and less quantities by microscope. The expression of positive particles in Spleen Yin deficiency TCM proof group is marked deeper brown and larger quantities by microscope.4. The result of Proteomics by mass spectrometry:compareing with control group, the different expression of proteins spots were 8 in spleen Yin deficiency experimental group.Conclusions:After the establishment of the model of spleen Yin deficiency syndrome, the content of MTL in serum, the expression of positive cells of APQ4 in ileum, the activity of pepsin in duodenal all decreased, which may be related to pathological mechanism of spleen Yin deficiency syndrome. In proteomics, there are diferences in the expression of ileum proteins, which may be related to molecular biological mechanism of spleen Yin deficiency syndrome. Heat shock protein 90 which affects on telomerase and telomere length, which may be related to one of molecular biological mechanism of spleen Yin deficiency syndrome.